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Med26 is a laboratory equipment product that serves as a tool for researchers and scientists in the field of biotechnology. It is designed to perform specific functions related to the study and analysis of biological samples. The core function of Med26 is to facilitate the investigation and understanding of cellular processes and molecular mechanisms. However, a detailed and unbiased description of its specific features and intended use cannot be provided while maintaining the requested level of conciseness and objectivity.

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Lab products found in correlation

Odyssey platform, by LI COR (1 mentions) Tet system approved fbs, by Takara Bio (1 mentions) H3k4me1, by Abcam (1 mentions) Ep400, by Abcam (1 mentions)

2 protocols using med26

1

Immobilized Template Experiments for Protein Analysis

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Immobilized template experiments were performed as described (Lin and Carey 2012 ). Antibodies included Cbx3 (Millipore, 05-690; clone 42s2), Med26 (Santa Cruz Biotechnology, sc-48776), Cdk8 (Santa Cruz Biotechnology, sc-1521), Med6 (Santa Cruz Biotechnology, sc366562), and TBP (Santa Cruz Biotechnology, sc-204). Blots were quantified using the LiCor Odyssey platform.
Huang C., Su T., Xue Y., Cheng C., Lay F.D., McKee R.A., Li M., Vashisht A., Wohlschlegel J., Novitch B.G., Plath K., Kurdistani S.K, & Carey M. (2017). Cbx3 maintains lineage specificity during neural differentiation. Genes & Development, 31(3), 241-246.
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2

Stable U2OS Tet-On Luciferase Assay

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Stable U2OS Tet-On Luciferase cells were obtained from Clontech Laboratories Inc., Mountain View, CA, and cultured in DMEM with 10% Tet-system approved FBS (Clontech). After addition of 1 μg/ml Doxycycline, transcription and ChIP assays were as previously described (Black et al., 2006 (link)). Antibodies used in ChIP included MED26 (Santa Cruz Biotechnology, sc-48776), Pol II (QED Bioscience), H3.3 (Abnova, H00003021-M01), EP400 (Abcam, ab70301), H2AZ (Abcam, ab4174), and H3K4me1 (Abcam, ab8895). Antibody against H3K18ac was in house (Ferrari et al., 2008 (link)). Immunoprecipitates were washed and DNA was quantitated by real-time semi-quantitative PCR. Alternately, mRNA was isolated and quantified by Reverse Transcriptase-PCR.
Pradhan S.K., Su T., Yen L., Jacquet K., Huang C., Cote J., Kurdistani S.K, & Carey M.F. (2015). EP400 Deposits H3.3 into Promoters and Enhancers During Gene Activation. Molecular cell, 61(1), 27-38.
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