Rb 9017 p0

Immunolocalization of Ki67, CD31, ESR1, PGR, p-IKK, p-STAT3, CD68, CD163, COX2, iNOS, MMP9 and p-AKT was determined in cross-sections (5 μm) of paraffin-embedded uterine sections using specific primary antibodies and a Vectastain Elite ABC Kit (Vector laboratories, Burlingame, CA, USA) or DyLight-conjugated secondary antibody (Jackson ImmunoResearch Lab, West Grove, PA, USA). Antibodies used in these analyses were: anti-CDH1 (1:120 dilution, 610181, BD Biosciences, San Jose, CA, USA), anti-Ki67 (1:200 dilution, 550609, BD Biosciences), anti-CD31 (1:100 dilution, ab28364, Abcam, Cambridge, MA, USA), anti-ESR1 (1:100 dilution, sc-542, Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-PGR (1:200 dilution, RB-9017-P0, Thermo Scientific, Rockford, IL, USA), p-IKK (1:150 dilution, 2697, Cell Signaling Technology, Danvers, MA, USA), p-STAT3 (1:50 dilution, 9145, Cell Signaling Technology), CD68 (1:300 dilution, ab955, Abcam), CD163 (1:300 dilution, ab126756, Abcam), COX2 (1:50 dilution, RM-9121, Thermo Scientific), iNOS (1:50 dilution, 610333, BD Biosciences), MMP9 (1:50 dilution, 3852, Cell Signaling Technology), and p-AKT (1:200 dilution, 3787, Cell Signaling Technology).