Human lung A549 (RRID:CVCL_0023), SK‐MES‐1 (RRID:CVCL_0630), H1299 (RRID:CVCL_0060), H1975 (RRID:CVCL_1511), and H460 (RRID:CVCL_0459) cancer cells were obtained from the American Type Culture Collection (ATCC: Manassa, VA). Cell lines were authenticated within the past 3 years using short tandem repeat DNA profiling and comparing the DNA sequences to the reference cell database, with an > 80% match being acceptable. Cells were assessed for mycoplasma contamination using the MycoAlert Mycoplasma Detection Kit (Lonza Group AG: Basel, Switzerland). Cell lines were cultured in DMEM (A549 and SK‐MES‐1), RPMI‐1640 (H1299 and H460) or ATCC‐modified RPMI‐1640 (H1975) with 10% FBS and 1% antibiotic‐antimycotic. All cells were maintained at 37 °C in a humidified 5% CO2 incubator. Cells were treated with canagliflozin (MedChemExpress LLC: Monmouth Junction, NJ) solubilized in dimethyl sulfoxide, cisplatin (Accord Healthcare Inc: Durham, NC), etoposide (Teva Canada Ltd: Toronto, Canada), radiation (6MV X‐rays as described [26 (link)]), Roxadustat (FG‐4592), BAY‐87‐2243, and IACS‐010759 (Cayman Chemical Company: Ann Arbor, MI).
Canagliflozin
Manufactured by MedChemExpress
Sourced in Canada, China, United States
Canagliflozin is a sodium-glucose co-transporter 2 (SGLT2) inhibitor, a class of medications used to lower blood glucose levels in individuals with type 2 diabetes. As a SGLT2 inhibitor, Canagliflozin works by reducing the reabsorption of glucose in the kidney, thereby increasing urinary glucose excretion.
Automatically generated - may contain errors
Lab products found in correlation
Roxadustat fg 4592, by Cayman Chemical (1 mentions)
Mycoalert mycoplasma detection kit, by Lonza (1 mentions)
H1299, by American Type Culture Collection (1 mentions)
H1975, by American Type Culture Collection (1 mentions)
Sk mes 1, by American Type Culture Collection (1 mentions)
Ampk inhibitor compound c, by MedChemExpress (1 mentions)
Dulbecco modified eagle medium (dmem), by MedChemExpress (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Empagliflozin, by MedChemExpress (1 mentions)
Dapagliflozin, by MedChemExpress (1 mentions)
Tofogliflozin, by Kowa (1 mentions)
3 protocols using canagliflozin
1
Evaluating Anti-Cancer Drugs in Lung Cell Lines
2
Modeling Stroke and Reperfusion in HT-22 Neurons
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The OGD/R model was used in the HT-22 mouse hippocampal neurons (Zhongqiaoxinzhou Biotechnology, Shanghai, China) to model the stroke and reperfusion as previously reported [21 (link)]. The HT-22 cells were cultured in Dulbecco's modified Eagle medium (DMEM, Gibco, USA) containing 10% fetal bovine serum (FBS, Sigma, USA) at 37° C, humidified 95% air and 5% CO2. For OGD, the medium was replaced with deoxygenated glucose-free medium (Gibco, USA), and incubated in a hypoxic incubator (95% N2 and 5% CO2) for 6 h to induce OGD injury. For OGD reoxygenation, the cells were then transferred to normal glucose-containing DMEM with different concentrations of canagliflozin or AMPK inhibitor compound C (CC, 10 μM, MedChem Express, Shanghai, China) and incubated in normal atmosphere for 24 h. After 24 h incubation, the HT-22 cells were collected for further experiments.
3
Quantification of SGLT2 Inhibitors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ipragliflozin, dapagliflozin, canagliflozin, and empagliflozin were obtained from Med-Chemexpress Co., Ltd. (New Jersey, USA). Tofogliflozin was kindly provided by Kowa Company Ltd. (Tokyo, Japan). Luseogliflozin was extracted from commercially available tablets (brand name: Lusefi®, Taisho Pharmaceutical Co., Ltd., Tokyo, Japan). Its purity was confirmed by 1H-NMR and acceptable for the standard analyte. All other reagents were of analytical grade and were used without further purification.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!