Fluorescence measurements of PLL-Dnc/protein mixtures in the presence of various concentrations of the additives were performed using a Cytation5 Imaging Reader (BioTek Instruments, Inc.). To each well of a 96-well microplate (96 Well Black Flat-Bottom Polystyrene NBS Microplates; Corning Inc.), 200 μL of a mixture of 4 μg/mL PLL-Dnc and 20 μg/mL HSA in 25 mM MOPS (pH 7.5) with or without one of the additives (0 -40 v/v% TFE; 0 -25 w/v% 1,6-hex; 0 -10 w/v% PEG; 0 -0.5 M Na2SO4; 0 -0.5 M NaSCN) was added. After incubation at 35°C for 10 min, the fluorescence intensity was recorded at 35°C for 10 min using excitation/emission wavelengths (λex/λem) of 340 nm/520 nm.
96 well black flat bottom polystyrene nbs microplates
Manufactured by Corning
Sourced in United States
The 96 Well Black Flat-Bottom Polystyrene NBS Microplates are a type of laboratory equipment designed for various applications in scientific research and analysis. These microplates feature a 96-well format with a black, flat-bottom polystyrene construction. The specific use and intended applications of this product are not provided within the scope of this factual and unbiased description.
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2 protocols using 96 well black flat bottom polystyrene nbs microplates
1
Fluorescence Assay of PLL-Dnc/Protein Interactions
2
Fluorescence Spectroscopy of Peptide-nGO Interactions
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Fluorescence measurements were performed on a Spectra max GEMINI XPS (Molecular devices, Sunnyvale, CA, USA). Solutions (200 μL) containing 20 nM P1 -FAM, 20 nM P2 -TAMRA, 20 nM P3 -Cy5, and 0–160 µg/mL nGO in PBS buffer (pH = 7.4) were prepared in each well of a 96-well plate (96-well black flat-bottom polystyrene NBS microplates; Corning Inc., Corning, NY, USA) using a PIPETMAX system (Gilson Inc., Middleton, WI, USA). After incubation (T = 30 °C, t = 10 min), fluorescence spectra were recorded at T = 30 °C using three different excitation wavelengths (P1 -FAM: λex = 480 nm, λem = 520–595 nm; P2 -TAMRA: λex = 530 nm, λem = 575–665 nm; P3 -Cy5: λex = 630 nm, λem = 660–750 nm). Binding isotherms were produced based on changes in the fluorescence intensity at λem = 519 nm (P1 -FAM), 579 nm (P2 -TAMRA), and 664 nm (P3 -Cy5).
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