Colistin sulfate powder was purchased from Sigma-Aldrich, and gamithromycin powder was obtained from Quality Control Chemicals Inc. (Walnut, CA, United States). Cefquinome was purchased from Qilu Animal Health Products Co., Ltd. (Jinan, China). Enrofloxacin, tilmicosin, tylosin tartrate, and florfenicol were obtained from the China Institute of Veterinary Drug Control (Beijing, China). Antibiotic stock solutions were prepared according to Clinical and Laboratory Standards Institute (CLSI) criteria before testing (Clinical, and Laboratory Standards Institute (CLSI), 2018 ). Mueller-Hinton agar was obtained from Qingdao Hope Bio-Technology Co., Ltd. (Qingdao, China). Sterile defibrinated sheep blood was purchased from Guangzhou Ruite Biotechnology Co., Ltd. (Guangzhou, China). Mueller-Hinton II broth (25.0 mg/L Ca2+ and 12.5 mg/L Mg2+; Becton, Dickinson and Company, Sparks, MD, United States) was used in all experiments.
Colistin sulfate powder
Manufactured by Merck Group
Sourced in United States
Colistin sulfate powder is a type of antibiotic medication used in laboratory settings. It is a white to slightly yellowish crystalline powder that is soluble in water. The core function of colistin sulfate powder is to serve as an antimicrobial agent for research and testing purposes.
Automatically generated - may contain errors
Lab products found in correlation
Mueller hinton 2 broth, by BD (1 mentions)
Mueller hinton agar (mha), by Hopebio (1 mentions)
Vitros 4600 system, by Ortho Clinical Diagnostics (1 mentions)
Camhb, by BD (1 mentions)
D glucose 6 phosphate disodium salt hydrate g 6 p, by Merck Group (1 mentions)
Mueller hinton agar (mha), by BD (1 mentions)
3 protocols using colistin sulfate powder
1
Antibiotic Susceptibility Testing Protocol
2
Colistin MIC Determination Protocol
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Duplicate MIC values to colistin were obtained by the recommended BMD method, according to CLSI/EUCAST (8, 20) , using in-house untreated 96-well sterile polystyrene microplates (Nunc, Roskilde, Denmark). Colistin sulfate powder (Sigma St.
Louis, Missouri, USA) was dissolved in CA-MHB (BD, Franklin Lakes, USA) (stock solution, 128 µg/ml) and log 2 dilutions were subsequently made to achieve a MIC range of 0.5 to 64 µg/ml. Cation levels (23.7 mg/L calcium and 11.2 mg/L magnesium) were verified by dry phase measurement (VITROS 4600 System, Ortho Clinical Diagnostics) after supplementation. The strains were considered to have acquired resistance to colistin when the MIC was higher than > 2 μg/ml, according to EUCAST standards (8) .
For colistin-susceptible mcr producers, MICs were confirmed by two additional reference dilution methods, a macro-dilution assay and the agar dilution technique (20) .
Louis, Missouri, USA) was dissolved in CA-MHB (BD, Franklin Lakes, USA) (stock solution, 128 µg/ml) and log 2 dilutions were subsequently made to achieve a MIC range of 0.5 to 64 µg/ml. Cation levels (23.7 mg/L calcium and 11.2 mg/L magnesium) were verified by dry phase measurement (VITROS 4600 System, Ortho Clinical Diagnostics) after supplementation. The strains were considered to have acquired resistance to colistin when the MIC was higher than > 2 μg/ml, according to EUCAST standards (8) .
For colistin-susceptible mcr producers, MICs were confirmed by two additional reference dilution methods, a macro-dilution assay and the agar dilution technique (20) .
3
Fosfomycin and Colistin Dilution Protocol
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Fosfomycin powder (96.5 % purity) was obtained from the National Institute for the Control of Pharmaceutical and Biological Products (NICPBP, Beijing, China). Colistin sulfate powder with a potency of 23 584 U mg -1 and D-glucose-6-phosphate disodium salt hydrate (G-6-P) were purchased from Sigma-Aldrich (St Louis, MO, USA). Mueller Hinton agar (MHA) and cationadjusted Mueller II broth (CA-MHB) were purchased from Becton, Dickinson (Franklin Lakes, NJ, USA). Fosfomycin and colistin were diluted in sterile water and used as prepared stock solutions of 2048 μg ml -1 and 1024 μg ml -1 , respectively.
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