Sulfobiotics hsip 1 da

OxyBlot protein oxidation detection kit was purchased from Merck Millipore (EMD Millipore, Billerica, MA, USA) and SulfoBiotics- HSip-1 DA from Dojindo Laboratories (Kumamoto, Japan). The generation of superoxide anion (O₂^•−) and ROS were detected using a commercially available kit from Enzo (Tokyo, Japan). Dimedone and acrolein were purchased from Tokyo Chemical Industry (Tokyo, Japan). The anti-cysteine sulfenic acid antibody was from Millipore (Burlington, MA, USA). Beta-cyano L-Alanine (BCA) was from Cayman Chemical (Ann Arbor, MI, USA). Anti-CSE, anti-CBS, and anti-3MPST antibodies were from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Anti-actin and anti-P-P38 antibodies, as well as horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG, were purchased from Cell Signaling, Inc. (Danvers, MA, USA). Alexa 680 Fluor C2 maleimide was from Thermo Scientific (Rockford, IL, USA). 17β-estradiol (E2), Sodium hydrosulfide hydrate (NaHS), L-cysteine hydrochloride, DL-Propargylglycine (PAG), glutathione (GSH), anti-Cx43, and all other chemicals were from Sigma (Tokyo, Japan).