Thermo scientific oxoid

Homogenates of 50mg liver tissue were filtered using a cell strainer (100 μm pore size; Corning, Tewksbury, MA 01876 USA) and serial dilutions were plated onto a blood agar plate (Thermo Scientific^™ Oxoid^™, Fisher Scientific, Wesel, Germany), brain heart infusion (BHI) agar (Thermo Scientific™), and water-blue metachrome-yellow lactose agar (acc. to Merck KGaA, Darmstadt, Germany). Additionally, Schaedler agar (Becton Dickinson GmbH, Heidelberg, Germany) was used for anaerobic growth examination in an anaerobic jar system (AnaeroGen^™; Thermo Scientific^™ Oxoid^™, Fisher Scientific, Oxford, OX14 4SD, UK) after 72 h of incubation at 37 °C. For enrichment purposes, each sample was inoculated to nutrient broth no. 2 containing 10% bovine serum and broth were streaked onto blood, BHI and Schaedler agar plate after 24 h incubation at 37 °C. Plates were examined after 24 and 48 h, as well as for BHI and Schaedler agar after 72 h at 10% CO₂. Morphologically diverse colonies were sub-cultivated. Pure cultures were identified using MALDI-TOF MS (Biotyper Version V3.3.1.0, Bruker Daltonics, Bremen, Germany) by the direct smear method and information provided in the DB7311 database. The MALDI-TOF results were supported by the Gram stain method and morphological properties of the bacteria.