The CRISPR activation plasmid specific for EphB2 was obtained from Santa Cruz Biotechnology, INC. AGS and HGC27 cells were transfected with 1 µg of the CRISPR activation plasmid specific for EphB2 (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA) in Opti-MEM Medium using Lipofectamine™ 3000 Transfection Reagent (ThermoFisher, USA) according to the manufacturer's instruction.
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