Cells were seeded in Costar six well plates (Corning, NY, USA). After reaching 50% confluence, culture media was substituted with Opti-MEM (GIBCO/Life Technologies, Carlsbad, CA). Cells were transfected with interference RNA (siRNA) [ECM-1 Silencer Select Pre-designed siRNA, 100–200nM, targeting exon 6, ECM-1 Silencer Select Pre-designed siRNA, 100–200nM, targeting exon 2, S100A4 Silencer Select Pre-designed siRNA, 100–200nM, (Ambion/Life Technologies, Carlsbad, CA) ] using Lipofectamine RNAiMAX (Invitrogen/Life Technologies, Carlsbad, CA). Silencer Select Negative Control #1 siRNA (Ambion/Life Technologies, Carlsbad, CA) was used as control siRNA. Adequate downregulation was assessed by western blotting.
Ecm 1 silencer select pre designed sirna
Manufactured by Thermo Fisher Scientific
The ECM-1 Silencer Select Pre-designed siRNA is a laboratory equipment product designed for gene silencing applications. It is a pre-designed, ready-to-use small interfering RNA (siRNA) that targets the ECM-1 gene.
Automatically generated - may contain errors
1 protocol using ecm 1 silencer select pre designed sirna
1
siRNA-Mediated Knockdown of ECM-1 and S100A4
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