Whatman grade 1 chr chromatography paper

Glucose oxidase (GOx) from Aspergillus niger, HRP, D-(+)- glucose, sodium acetate anhydrate, sodium phosphate monobasic, and acetic acid were purchased from Sigma Aldrich Co. (Saint, Louis, MO). Sodium phosphate dibasic and KI were purchased from Fischer Scientific Co. (Hampton, NH). Whatman Grade 1 CHR chromatography paper was purchased from GE Healthcare Life Sciences (Pittsburgh, PA).

Glucose oxidase (GOx) from Asperillus niger was a gift from Fitzgerald Industries International (Acton, MA). D-(+)-glucose, acetylcholinesterase (AChE) from Electrophorous electricus (electric eel) (14.6 mg solid, 137 U/mg), acetylthiocholine iodide (ATC), 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB), and sodium phosphate monobasic were purchased from Sigma Aldrich Co. (St. Louis, MO). Sodium phosphate dibasic and KI were purchased from Fischer Scientific Co. (Hampton, NH). Whatman Grade 1 CHR chromatography paper was purchased from GE Healthcare Life Sciences (Pittsburgh, PA).
PBS buffer (0.1 M, pH 6.0) was prepared by addition of 87.7 mL of sodium phosphate monobasic (0.2 M) and 12.3 mL of sodium phosphate dibasic (0.2 M). GOx (120 U/mL) was prepared with phosphate-buffered saline (PBS) (pH 6.0). A HRP solution was prepared with PBS at 30 U/mL. KI (1.0 M) was prepared with DI water. Various concentrations of glucose were made in DI water (0.5, 1 -4 mM). DTNB (5 mg/mL), various concentrations of ATC (0, 0.5, 1 -14 mM) and AChE (0.18 U/mL) were prepared in PBS buffer (pH 6.0). A solution of AChE:DTNB (1:1) was prepared fresh prior to the ATC assays.