The whole brains were thawed and homogenized in 2.5 mL 50 mM Tris HCl buffer (pH 7.4) with 0.5% Triton, using a glass Teflon homogenizer (15 strokes, 900 rpm; Eurostar Power-B, IKA-Werke). Duplicates of 100 and 250 µl were transferred to glass scintillation vials (Perkin Elmer, 20 mL) and 15 mL Hionic-Fluor (Perkin Elmer) was added. The samples were shaken for 60 s and stored in darkness for 30 min before radioactivity was counted in a liquid scintillation analyzer (Tri-Carb 2810TR; PerkinElmer). Brain radioactivity was expressed as the percentage of the total injected radioactivity left in the brain.
Eurostar power b
Manufactured by IKA Group
The Eurostar Power-B is a laboratory stirrer from IKA Group. It features electronic speed control and a digital display to monitor the stirring speed.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using eurostar power b
1
Quantifying Brain Radioactivity
2
Synthesis and Characterization of ALPCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
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previously synthesized LGPCs (1 mol), a phase transfer catalyst (tetrabutylammonium
hydrogen sulfate, TBAHS; tetrabutylammonium bromide, TBAB; tetrabutylammonium
iodide, TBAI; cetyltrimethylammonium bromide, CTAB; methyltriphenylphosphonium
bromide, MTPB; phenyltrimethylammonium tribromide, PTMTB; or tetrabutylphosphonium
bromide, TBPB) (0.003 mol), NaOH (1.5 mol), and the respective alkyl
bromide (1-bromododecane, 1-bromotetradecane, 1-bromohexadecane, or
1-bromooctadecane) were carefully added to a glass reactor with five
joints of 24 × 40N. The solid mixture was heated
at 100 °C under constant agitation with the aid of a mechanical
stirrer Eurostar Powerb IKAWERKE. After 72 h and reaction completion
(follow-up through TLC analysis), the final mixture was washed three
times with brine solution. Product (ALPC) liquid–liquid extraction
was performed with hexane. Excess water was then removed through Na2SO4 addition followed by the salt filtration. Finally,
the exceeding solvent removal was later performed in vacuo. The major ALPC [1-O-dodecyl-2-O-acyl-sn-glycero-3-phosphocholine (ALPC12), 1-O-tetradecyl-2-O-acyl-sn-glycero-3-phosphocholine (ALPC14), 1-O-hexadecyl-2-O-acyl-sn-glycero-3-phosphocholine (ALPC16),
and 1-O-octadecyl-2-O-acyl-sn-glycero-3-phosphocholine (ALPC18)] product yields are
summarized inTable 3 .
previously synthesized LGPCs (1 mol), a phase transfer catalyst (tetrabutylammonium
hydrogen sulfate, TBAHS; tetrabutylammonium bromide, TBAB; tetrabutylammonium
iodide, TBAI; cetyltrimethylammonium bromide, CTAB; methyltriphenylphosphonium
bromide, MTPB; phenyltrimethylammonium tribromide, PTMTB; or tetrabutylphosphonium
bromide, TBPB) (0.003 mol), NaOH (1.5 mol), and the respective alkyl
bromide (1-bromododecane, 1-bromotetradecane, 1-bromohexadecane, or
1-bromooctadecane) were carefully added to a glass reactor with five
joints of 24 × 40N. The solid mixture was heated
at 100 °C under constant agitation with the aid of a mechanical
stirrer Eurostar Powerb IKAWERKE. After 72 h and reaction completion
(follow-up through TLC analysis), the final mixture was washed three
times with brine solution. Product (ALPC) liquid–liquid extraction
was performed with hexane. Excess water was then removed through Na2SO4 addition followed by the salt filtration. Finally,
the exceeding solvent removal was later performed in vacuo. The major ALPC [1-O-dodecyl-2-O-acyl-sn-glycero-3-phosphocholine (ALPC12), 1-O-tetradecyl-2-O-acyl-sn-glycero-3-phosphocholine (ALPC14), 1-O-hexadecyl-2-O-acyl-sn-glycero-3-phosphocholine (ALPC16),
and 1-O-octadecyl-2-O-acyl-sn-glycero-3-phosphocholine (ALPC18)] product yields are
summarized in
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