Ht29 cell lines
The HT29 cell line is an immortalized human colorectal adenocarcinoma cell line. It is a widely used in vitro model for the study of intestinal epithelial cells and colorectal cancer research.
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4 protocols using ht29 cell lines
Cultivation of Human and Murine Colon Cancer Cell Lines
Nanoemulsion Formulation and Cell Lines
Lecithin was obtained from Alfa Aesar (Karlsruhe, Germany). Sodium caseinate (NaCas) (≥92% purity) was from Acros Organics (Geel, Belgium). The Caco-2 cell line was purchased from the European Collection of Cell Cultures (ECACC 86010202) and the human monocyte THP-1 (ATCCTIB-202) and the human colon adenocarcinoma HT-29 cell lines (ATCCHTB-38) were purchased from American Type Culture Collection. This latter cell line was differentiated to HT-29-MTX following the protocol described by Guri et al. (2013) . Tissue culture plastics were sourced from Sarstedt Ltd. (Wexford, Ireland). CellTiter 96 AQueus One Solution reagent was purchased from Promega (MyBio, Kilkenny, Ireland). Milli-Q water was used to prepare all nanoemulsions. All other chemicals were sourced from Sigma-Aldrich (Ireland) unless specified otherwise.
HT-29 Cell Culture Protocol
Curcumin Modulates Transcriptional Profiles
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