We originally purchased SW620, HT29 cell lines of human colon cancer and MC38 murine colon cancer cell line, which will be utilized in following experiments, from the American Type Culture Collection (Manassas, VA), and the cells were cultivated in DMEM medium according to the Defense Technical Information Center recommendation (DTIC) in addition of 10% fetal bovine serum (FBS, Gibico, Life Technology, Austria), 1% penicillin/streptomycin (PS) in a humidified 5% (v/v) atmosphere of CO2 at 37 °C.
Ht29 cell lines
Manufactured by American Type Culture Collection
Sourced in Austria, United States
The HT29 cell line is an immortalized human colorectal adenocarcinoma cell line. It is a widely used in vitro model for the study of intestinal epithelial cells and colorectal cancer research.
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Lab products found in correlation
Sw620, by American Type Culture Collection (1 mentions)
Caco 2 cell line, by European Collection of Cell Cultures (1 mentions)
Lecithin, by Thermo Fisher Scientific (1 mentions)
β carotene, by Merck Group (1 mentions)
Atcc htb 38, by American Type Culture Collection (1 mentions)
12 mm round coverslips, by Warner Instruments (1 mentions)
4 protocols using ht29 cell lines
1
Cultivation of Human and Murine Colon Cancer Cell Lines
2
Nanoemulsion Formulation and Cell Lines
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Corn oil (Mazola, ACH Food Companies Inc., Memphis, TN) was purchased from a local market. β-carotene (synthetic, ≥93% (UV), powder) was sourced from Sigma-Aldrich (Ireland).
Lecithin was obtained from Alfa Aesar (Karlsruhe, Germany). Sodium caseinate (NaCas) (≥92% purity) was from Acros Organics (Geel, Belgium). The Caco-2 cell line was purchased from the European Collection of Cell Cultures (ECACC 86010202) and the human monocyte THP-1 (ATCCTIB-202) and the human colon adenocarcinoma HT-29 cell lines (ATCCHTB-38) were purchased from American Type Culture Collection. This latter cell line was differentiated to HT-29-MTX following the protocol described by Guri et al. (2013) . Tissue culture plastics were sourced from Sarstedt Ltd. (Wexford, Ireland). CellTiter 96 AQueus One Solution reagent was purchased from Promega (MyBio, Kilkenny, Ireland). Milli-Q water was used to prepare all nanoemulsions. All other chemicals were sourced from Sigma-Aldrich (Ireland) unless specified otherwise.
Lecithin was obtained from Alfa Aesar (Karlsruhe, Germany). Sodium caseinate (NaCas) (≥92% purity) was from Acros Organics (Geel, Belgium). The Caco-2 cell line was purchased from the European Collection of Cell Cultures (ECACC 86010202) and the human monocyte THP-1 (ATCCTIB-202) and the human colon adenocarcinoma HT-29 cell lines (ATCCHTB-38) were purchased from American Type Culture Collection. This latter cell line was differentiated to HT-29-MTX following the protocol described by Guri et al. (2013) . Tissue culture plastics were sourced from Sarstedt Ltd. (Wexford, Ireland). CellTiter 96 AQueus One Solution reagent was purchased from Promega (MyBio, Kilkenny, Ireland). Milli-Q water was used to prepare all nanoemulsions. All other chemicals were sourced from Sigma-Aldrich (Ireland) unless specified otherwise.
3
HT-29 Cell Culture Protocol
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The HT-29 cell lines used in this work were obtained from the American Type Culture Collection (Rockville, MD) and were cultured at 37°C under 5% CO2 in RPMI 1640 medium supplemented with antibiotics (100 U/ml of penicillin and 100μg/ml of streptomycin) and 10% heat-inactivated fetal bovine serum. After the cells had grown to confluence, they were replated onto 12-mm round coverslips (Warner Instruments Inc., Hamden, CT) and incubated for at least 24 h before use for cytosolic Ca2+ concentration([Ca2+]cyt).
4
Curcumin Modulates Transcriptional Profiles
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Human hepatocellular carcinoma HepG2 and human colorectal cancer HT29 cell lines (American Type Culture Collection [ATCC], Rockville, MD, USA) were treated in triplicate with 20 μM of curcumin dissolved in dimethyl sulfoxide. These samples were then submitted to Genometry, Inc. (Cambridge, MA, USA) for L1000 microarray profiling. The microarray gene expression profiling results were classified according to the up- and down-regulated gene signatures, which were subsequently used to query the C-Map [3 (link)] and CLUE [4 (link)] databases for analysis of the gene expression of curcumin. Additional details are listed in Supplementary materials .
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