Nmri nu immunodeficient mice

Adherent (1 × 10⁶) or spheroidal (1 × 10⁵) cultures of GCTB stromal Pat-2, Pat-3 and Pat-8 cells were transplanted in 50% Matrigel/PBS subcutaneously to the left and right flanks of 5-week-old female NMRI-nu immunodeficient mice (Janvier Labs, Saint-Berthevin, France) in a total volume of 100 μl. The tumor engraftment and tumor size were measured and analyzed as described previously.^{45 (link)} The animal experiments were carried out in the animal facilities of the University of Heidelberg after approval by the authorities (Regierungspräsidium, Karlsruhe, Germany).

NMRI‐nu immunodeficient mice were obtained from Janvier Laboratory. Experiments were performed on 7–8 weeks old female mice after 1 week of acclimation in animal facility of Curie Institute. Mice were housed under a controlled temperature and 12 h/12 h light–dark cycle with access to food and water ad libitum in conventional animal facility. For the animal welfare, mice are maintained in social groups with enrichment. Animal care and use for this study were performed in accordance with the recommendations of the European Community (2010/63/UE) for the care and use of laboratory animals. Experimental procedures were specifically approved by the ethics committee of the Institut Curie CEEA‐IC #118 (Authorization 02383.02 given by National Authority) in compliance with the international guidelines.