All reagents were obtained from Sigma–Aldrich (St. Louis, MO, USA) unless otherwise indicated. Cell culture medium was obtained from Thermo Fisher Scientific (Waltham, MA, USA) or Santa Cruz Biotechnology (Santa Cruz, CA, USA). Penicillin/streptomycin was obtained from Thermo Fisher Scientific. Fetal bovine serum (FBS) was purchased from Thermo Fisher Scientific. EquaFETAL® bovine serum (EBS) was purchased from Atlas Biologicals, Inc. (Fort Collins, CO, USA). Hank’s Balanced Salt Solution (HBSS) was purchased from Thermo Fisher Scientific. Monoclonal antibodies to FKBP51 were described previously.11 (link) TRPC4 antibody was obtained from Alomone Labs (Jerusalem, Israel). Polyclonal antibody for PP5C was previously described.12 (link) β-actin antibody was purchased from Santa Cruz Biotechnology.
Trpc4 antibody
Manufactured by Alomone
Sourced in Israel
The TRPC4 antibody is a specific and sensitive tool used to detect the TRPC4 protein, which is a member of the transient receptor potential (TRP) family of cation channels. The TRPC4 antibody can be used in various applications, such as Western blotting, immunohistochemistry, and immunoprecipitation, to study the expression and localization of the TRPC4 protein in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
β actin antibody, by Santa Cruz Biotechnology (1 mentions)
Cell culture medium, by Thermo Fisher Scientific (1 mentions)
Hank s balanced salt solution hbss, by Thermo Fisher Scientific (1 mentions)
Bmpr 2 antibody, by BD (1 mentions)
Pluronic f 127, by Thermo Fisher Scientific (1 mentions)
2 protocols using trpc4 antibody
1
FKBP51, TRPC4, and PP5C Antibody Characterization
2
Investigating BMP4 Signaling Pathways
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Unless otherwise noted, all reagents were obtained from Sigma-Aldrich. All antibodies were obtained as follow: TRPC1, 6 antibodies (abcam), TRPC4 antibody (Alomone Laboratories), phospho-Smad1/5/8, phospho-ERK1/2, phospho-p38MAPK, ERK1/2, Smad5 and p38MAPK antibodies (Cell Signaling Technology), BMPRIa antibody (Proteintech Group), BMPRIb antibody (Invitrogen), ALK2 antibody (Novus), BMPRII antibody (BD), ActR2a and ActR2b antibodies (Abbiotec), α-actin antibody (Sigma-Aldrich).
Stock solutions of rhBMP4 at 50 µg/ml were made in 4 mM HCl containing 0.1% BSA. Stock solutions 30 mM of CPA and 5 mM nifedipine were made in dimethyl sulfoxide (DMSO) and 200 mM of MnCl2 was dissolvd in deionized water. Fura-2 AM was prepared as a 2.5 mM stock solution in DMSO containing 20% pluronic F-127 (Invitrogen).
Stock solutions of rhBMP4 at 50 µg/ml were made in 4 mM HCl containing 0.1% BSA. Stock solutions 30 mM of CPA and 5 mM nifedipine were made in dimethyl sulfoxide (DMSO) and 200 mM of MnCl2 was dissolvd in deionized water. Fura-2 AM was prepared as a 2.5 mM stock solution in DMSO containing 20% pluronic F-127 (Invitrogen).
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