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Crl 2256

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The CRL-2256 is a cell line derived from a human embryonic kidney cell. It is commonly used for cell biology research and assay development. The core function of this product is to provide a renewable source of human kidney cells for in vitro studies.

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Lab products found in correlation

Rbl 2h3, by American Type Culture Collection (3 mentions) Rpmi 1640 medium, by Nacalai Tesque (1 mentions) Echistatin, by R&D Systems (1 mentions) Pcs 201 012, by American Type Culture Collection (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle s medium, by Welgene (1 mentions) Crl 1573, by American Type Culture Collection (1 mentions) Streptomycin, by Welgene (1 mentions) Raw264, by American Type Culture Collection (1 mentions) Penicillin, by Welgene (1 mentions) Tib 71, by American Type Culture Collection (1 mentions)

7 protocols using crl 2256

1

Maintaining Rat Basophilic Leukemia Cells

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Rat basophilic leukemia-2H3 (RBL-2H3) cell line [35] (link) was purchased from ATCC (CRL-2256). RBL-2H3 cells were maintained in adherent cultures in RPMI 1640 medium (Nacalai tesque, Kyoto, Japan) supplemented with 10% FBS in a humidified atmosphere of 5% CO2 at 37°C.
Nigorikawa K., Hazeki K., Guo Y, & Hazeki O. (2014). Involvement of Class II Phosphoinositide 3-Kinase α-Isoform in Antigen-Induced Degranulation in RBL-2H3 Cells. PLoS ONE, 9(10), e111698.
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2

Culturing and Differentiating Leukemia Cell Lines

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Human promyeloblast leukemia HL-60 cells were cultured in RPMI 1640 medium supplemented with 10% heat-inactivated FBS, 100 Mg/ml streptomycin, 100 U/ml penicillin. For HL-60 differentiation (dHL-60), DMSO was added to a final concentration of 1.3% (v/v) and the cells were cultured for an additional 6 days. Rat basophils leukemia cells (RBL-2H3, ATCC® CRL-2256™) were stably transfected with human FPR2 (RBL-FPR2 cells) and cultured in DMEM supplemented with 20% FBS, 100 Mg/ml streptomycin, 100 U/ml penicillin, and 250 Mg/ml G418 (He et al., 2000 (link)). COSphox cells were cultured in DMEM supplemented with 10% FBS, 100 Mg/ml streptomycin, 100 U/ml penicillin, 0.5mg/ml G418, 0.8 mg/ml neomycin and 0.2 mg/ml hygromycin (Price et al., 2002 (link)).
Wang H., Peng X., Ge Y., Zhang S., Wang Z., Fan Y., Huang W., Qiu M, & Ye R.D. (2020). A Ganoderma-Derived Compound Exerts Inhibitory Effect Through Formyl Peptide Receptor 2. Frontiers in Pharmacology, 11, 337.
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3

Rat mast cell line RBL-2H3 culture protocol

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Rat mucosal mast cell line (RBL-2H3; CRL-2256™, ATCC, Manassas, VA, USA) was maintained in ATCC-formulated Eagle’s minimum essential medium (30-2003, ATCC) supplemented with 15% fetal calf serum and 1% penicillin/streptomycin (Gibco). Normal human dermal fibroblast (NHDF; PCS-201-012™, ATCC, Manassas, VA, USA) were maintained in Dulbecco’s modified Eagle’s medium (Welgene, Daegu, Korea) supplemented with 10% fetal calf serum and 1% penicillin/streptomycin (Gibco). Cells were maintained at 37 °C, under 5% CO2.
For treatment with LIFE, echistatin (3202, R&D system), and ellagic acid (5070, ChromaDex™), RBL-2H3 cells (passage 3–7) at 90% confluence were treated with substances for 24 h.
Yeom M., Ji H., Shin J., Cho E., Ryu D.H., Park D, & Jung E. (2022). The Alleviating Effect of Lagerstroemia indica Flower Extract on Stretch Marks through Regulation of Mast Cells. Molecules, 27(4), 1274.
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4

Inhibition of Grass Pollen Allergen-Induced Degranulation

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The capacity of TGFβ1‐mim to inhibit Phl p 5‐induced degranulation in murine RBL‐2H3 (muRBL) cells (ATCC® CRL2256™) was performed as described elsewhere.21 Humanized RBL‐2H3 (huRBL) cells transfected with the cDNA encoding the human FcɛRI were tested with nine sera from grass pollen‐allergic patients, as described elsewhere.22 Experiments using anonymized human serum samples were approved by the local Ethics Committee of the Medical University and General Hospital of Vienna (no. EK1263/2014), and informed written consent was obtained from all study participants. A detailed description is provided in the Appendix S1.
Araujo G.R., Aglas L., Vaz E.R., Machado Y., Huber S., Himly M., Duschl A., Goulart L.R, & Ferreira F. (2019). TGFβ1 mimetic peptide modulates immune response to grass pollen allergens in mice. Allergy, 75(4), 882-891.
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5

Overexpression of Human CMKLR1 in Cell Lines

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HeLa cells (CRM-CCL2, ATCC, Manassas, VA, USA) were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% heat-inactivated fetal bovine serum. Full-length human CMKLR1 cDNA was subcloned into the expression plasmids pcDNA3.1(+) for transfection into HEK 293 and SFFV.neo for transfection into RBL-2H3. A CMKLR1-GFP fusion construct was also prepared, by in-frame fusion of full-length CMKLR1 coding sequence to that of enhanced green fluorescent protein (minus the first codon for methionine), and subcloned into the EcoRI site of the pcDNA3.1 vector. Site-directed mutagenesis was performed on the cDNA of human CMKLR1 and the mutations were verified by DNA sequencing. HeLa cells were transfected with the expression plasmids using Lipofectamine 2000 according to manufacturer’s instructions. The cells were incubated at 37°C for 24–36 h after transfection prior to performing the functional assays.
To generate stable transfectants, the rat basophilic leukemia RBL-2H3 cell line (CRL-2256, ATCC) and the human kidney epithelial HEK 293 cell line (CRL-1573, ATCC) were transfected with the CMKLR1-SFFV.neo and CMKLR1-pcDNA3.1 plasmids, respectively, using Lipofectamine 2000. Stable transfectants were selected with 500 µg/mL G418 (Life Technologies) and were maintained in the presence of 250 µg/mL G418.
Zhou J.X., Liao D., Zhang S., Cheng N., He H.Q, & Ye R.D. (2014). Chemerin C9 peptide induces receptor internalization through a clathrin-independent pathway. Acta Pharmacologica Sinica, 35(5), 653-663.
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6

Cell Culture Maintenance Protocol

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The RAW 264.7 (mouse macrophages, ATCC; TIB-71) and RBL-2H3 (rat basophilic leukemia cells, ATCC; CRL-2256) cell lines were obtained from the American Type Culture Collection (Manassas, VA, USA). The human keratinocyte (HaCaT) cell line was obtained from the Korean Cell Bank. The cell lines were maintained in DMEM or RPMI 1640 media supplemented with 10% FBS, 100 units/mL penicillin, and 100 µg/mL streptomycin (Welgene, Republic of Korea) and incubated in a 5% CO2 humidified incubator at 37 °C.
Lee J.H., Lim J.Y., Jeon Y.D., Yun D.H., Lee Y.M, & Kim D.K. (2023). Wheatgrass-and-Aronia-Mixed Extract Suppresses Immunoglobulin E-Mediated Allergic Reactions In Vitro and In Vivo. International Journal of Molecular Sciences, 24(15), 11979.
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7

Culturing RBL-2H3 Rat Basophilic Cells

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RBL-2H3 rat basophilic leukaemia (CRL-2256™) cell lines were obtained from American Type Culture Collection (ATCC) (Rockville, MD, USA). RBL-2H3 cells was cultured in EMEM conditioned with 15% heat inactivated FBS (HI-FBS) at 37 °C in a humidified incubator (5% CO2, 95% air).
Richard E.J., Murugan S., Bethapudi B., Illuri R., Mundkinajeddu D, & Chinampudur Velusami C. (2017). Is Andrographis paniculata extract and andrographolide anaphylactic?. Toxicology Reports, 4, 431-437.
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