Napqi

Manufactured by Merck Group

Sourced in United States

NAPQI is a laboratory reagent used in the analysis and detection of certain compounds. It serves as a key component in various analytical methods, providing a reliable and accurate means of identifying and quantifying specific substances. The core function of NAPQI is to facilitate these analytical processes, allowing researchers and scientists to obtain valuable data and insights.

Automatically generated - may contain errors

Lab products found in correlation

Pierce bca protein assay kit, by Thermo Fisher Scientific (6 mentions) Primescript rt master mix, by Takara Bio (3 mentions) Sybr premix ex taq, by Takara Bio (3 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Lamin b, by Cell Signaling Technology (2 mentions) Enhanced chemiluminescence kit, by Merck Group (2 mentions) H2dcfda, by Thermo Fisher Scientific (2 mentions) Whole cell protein extraction kits, by Merck Group (2 mentions) Trizol reagent, by Thermo Fisher Scientific (2 mentions) Rpmi 1640, by Thermo Fisher Scientific (2 mentions) Cpt1a, by Cell Signaling Technology (1 mentions) Ampkα1, by Cell Signaling Technology (1 mentions) Phospho gsk3β, by Cell Signaling Technology (1 mentions) Ne per nuclear cytoplasmic extraction reagents, by Thermo Fisher Scientific (1 mentions) Phospho ampk α1, by Cell Signaling Technology (1 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions) Anti actin, by Cell Signaling Technology (1 mentions) Gsk3β, by Cell Signaling Technology (1 mentions) Compound c, by Merck Group (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)

7 protocols using napqi

Detailed Molecular Mechanisms of Oxidative Stress Response

Check if the same lab product or an alternative is used in the 5 most similar protocols

API (purity >98.5%) was purchased from Meilune (Dalian, China). Kits for ROS, MDA, and MPO were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Lipofectamine RNAiMAX, 2′-7′-dichlorodihydrofluorescein diacetate (H₂DCFDA), RPMI1640, and fetal bovine serum (FBS) were purchased from Life Technology (Carlsbad, CA, United States). NE-PER nuclear, cytoplasmic extraction reagents, and a Pierce BCA Protein Assay Kit were purchased from Thermo Fisher Scientific (Waltham, MA, United States). A whole-cell protein extraction and enhanced chemiluminescence kits were obtained from Millipore (Darmstadt, Germany). PrimeScript RT Master Mix and SYBR Premix Ex Taq were purchased from TaKaRa (Shiga, Japan). Antibodies for immunoblotting, including anti-actin, -CPT1A, -Phospho -AMPKα1, -AMPKα1, -Phospho -GSK-3β, -GSK-3β, -NRF2, and -LaminB were purchased from Cell Signaling Technology (Danvers, MA, United States). Methanol and acetonitrile (HPLC grade) were purchased from Thermo Fisher Chemicals (Waltham, MA, United States). APAP, NAPQI, and compound C were purchased from Sigma-Aldrich (St. Louis, MO, United States). Fenclonine was purchased from Aladdin (Shanghai, China).

Zhang J., Liang X., Li J., Yin H., Liu F., Hu C, & Li L. (2020). Apigenin Attenuates Acetaminophen-Induced Hepatotoxicity by Activating AMP-Activated Protein Kinase/Carnitine Palmitoyltransferase I Pathway. Frontiers in Pharmacology, 11, 549057.

+ Open protocol

+ Expand

Apigenin Alleviates Acetaminophen-Induced Liver Injury

Check if the same lab product or an alternative is used in the 5 most similar protocols

Apigenin (purity >99.5%) was purchased from Shanghai Hitsanns Co., Ltd (Shanghai, China). Kits for alanine aminotransferase (ALT), aspartate aminotransferase (AST), malondialdehyde (MDA), myeloperoxidase (MPO), and glutathione (GSH) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). H2DCFDA, RPMI1640, and fetal bovine serum (FBS) were purchased from Life Technology (Carlsbad, CA, USA). A Pierce BCA Protein Assay Kit was purchased from Thermo Fisher Scientific (Waltham, MA, USA). EX-527 was purchased from Sigma-Aldrich (St. Louis, MO, USA). Whole-cell protein extraction kits and enhanced chemiluminescence kits were obtained from Millipore (Darmstadt, Germany). Antibodies for immunoblotting, including β-actin (#4970), Lamin B (#13435), SIRT1 (#8469), p53 (#2524), ac382-p53 (#2525), NRF2 (#12721), and p65 (#8242) were purchased from Cell Signaling Technology (Danvers, MA, USA; all 1: 1,000 dilutions). Enzyme-linked immunosorbent assay (ELISA) kits were purchased from RapidBio (West Hills, CA, USA). TRIzol reagent was purchased from Life Technology (Carlsbad, CA, USA). PrimeScript RT Master Mix and SYBR Premix Ex Taq were purchased from TaKaRa (Shiga, Japan). APAP, NAPQI, 3-(4,5-dimethyl-thiazol-2-yl)2,5-diphenyltetrazolium bromide (MTT), and other reagents were purchased from Sigma-Aldrich unless otherwise indicated.

Zhao L., Zhang J., Hu C., Wang T., Lu J., Wu C., Chen L., Jin M., Ji G., Cao Q, & Jiang Y. (2020). Apigenin Prevents Acetaminophen-Induced Liver Injury by Activating the SIRT1 Pathway. Frontiers in Pharmacology, 11, 514.

+ Open protocol

+ Expand

Evaluation of Hepatoprotective Agents

Check if the same lab product or an alternative is used in the 5 most similar protocols

EtPy was purchased from Alfa Aesar (Ward Hill, MA) and Sigma-Aldrich (St. Louis, MO). Sodium pyruvate (PyA) was obtained from Nacalai Tesque (Kyoto, Japan). Sodium phosphopyruvate was kindly donated by Ube Kousan (Yamaguchi, Japan). APAP, N-acetyl cyctein (NAC) and NAPQI were purchased from Sigma-Aldrich. A cell counting kit was obtained from Dojindo Laboratories (Kumamoto, Japan). An annexin V-FITC apoptosis detection kit was purchased from R&D Systems (Minneapolis, MN). All other reagents and solvents were of reagent grade. Deionized and distilled bio-pure grade water was used in the study.

Nagatome M., Kondo Y., Kadowaki D., Saishyo Y., Irikura M., Irie T, & Ishitsuka Y. (2018). Ethyl pyruvate attenuates acetaminophen-induced liver injury and prevents cellular injury induced by N-acetyl-p-benzoquinone imine. Heliyon, 4(2), e00521.

+ Open protocol

+ Expand

Hepatoprotective Effects of Flavonoid Compound

Check if the same lab product or an alternative is used in the 5 most similar protocols

FST of 98% purity was purchased from Shanghai Hitsanns Co., Ltd (Shanghai, China). Kits for the analysis of alanine/aspartate aminotransferases (ALT/AST), malondialdehyde (MDA), myeloperoxidase (MPO), and GSH were obtained from Nanjing Jiancheng (Nanjing, China). H2DCFDA, RPMI1640, and fetal bovine serum (FBS) were obtained from Life Technology (Carlsbad, CA, USA). The Pierce^® BCA Protein Assay Kit was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Whole cell protein extraction kits and enhanced chemiluminescence kits were obtained from Millipore (Darmstadt, Germany). Antibodies for immunoblotting including β-actin (#4970), LC3 (#2775), p62 (#88588), ATG5 (#2630), caspase-1 (#3866), IL-1β (#12242) were purchased from Cell Signaling Technology (Danvers, MA, USA) (all 1:1,000 dilutions). Enzyme-linked immunosorbent assay (ELISA) kits were purchased from RapidBio (West Hills, CA, USA). TRIzol reagent was purchased from Life Technology (Carlsbad, CA, USA). PrimeScript^® RT Master Mix and SYBR^® Premix Ex Taq^™ were purchased from Takara (Shiga, Japan). APAP, NAPQI, MTT, and other reagents were obtained from Sigma-Aldrich (St. Louis, MO, USA) unless otherwise indicated.

Zhang J., Zhao L., Hu C., Wang T., Lu J., Wu C., Chen L., Jin M., Hu H., Ji G., Cao Q, & Jiang Y. (2020). Fisetin Prevents Acetaminophen-Induced Liver Injury by Promoting Autophagy. Frontiers in Pharmacology, 11, 162.

+ Open protocol

+ Expand

Enzyme Kinetics of Redox Regulators

Check if the same lab product or an alternative is used in the 5 most similar protocols

Purified rat liver TrxR1, recombinant human Trx1 and glutathione
reductase (GR), recombinant E. coli Trx1, bovine glutathione
peroxidase (GPx), insulin, NADPH, 5,5′-dithiobis(2-nitrobenzoic acid)
(DTNB), APAP, NAPQI, menadione (2-methyl-1,4-napthoquinone), reduced glutathione
(GSH), oxidized glutathione (GSSG), phosphatase inhibitors (catalog no. P2850,
which contains microcystinLR, cantharidin, and
(−)-p-bromotetramisole) and protease inhibitor cocktail
(catalog no. P2714, which contains 4-(2-aminoethyl)benzenesulfonyl fluoride,
E-64, bestatin, leupeptin, aprotinin, and EDTA) were purchased from
Sigma-Aldrich (St. Louis, MO). Sodium aurothiomalate hydrate was from Aldrich
(Milwaukee, WI). Human recombinant TrxR mutant enzyme (Sec498Cys) was from
AbFrontier (Seoul, Korea). Pooled human liver microsomes (HLM) and recombinant
human P450s 1A2, 2E1, and 3A4 were purchased from BD Genetest (Woburn, MA).
N-(Biotinoyl)-N′-(iodoacetyl)
ethylenediamine (BIAM) was from Molecular Probes (Eugene, OR) and horseradish
peroxidase (HRP)-conjugated streptavidin from GE Healthcare (Piscataway, NJ).
Enhanced chemiluminescence (ECL) immunoblot detection reagents were from Thermo
Scientific (Rockford, IL).

Jan Y.H., Heck D.E., Dragomir A.C., Gardner C.R., Laskin D.L, & Laskin J.D. (2014). Acetaminophen Reactive Intermediates Target Hepatic Thioredoxin Reductase. Chemical Research in Toxicology, 27(5), 882-894.

+ Open protocol

+ Expand

Characterization of Quinone Compounds and Environmental Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

1,2-NQ (97.4% purity determined by high-performance liquid chromatography (HPLC)), 1,4-NQ (98% purity determined by gas chromatography (GC)), 5,8-dihydroxy-1,4-NQ (79.8% purity determined by HPLC), 1,4-BQ (98% purity determined by iodometric titration), tertbutyl-1,4-benzoquinone (TBQ, 98% purity determined by GC), 2-hydroxy-1,4-NQ (99.9% purity determined by HPLC), and APAP were purchased from Tokyo Chemical Industry (Tokyo, Japan). 2-Methyl-1,4-NQ (98% purity determined by HPLC) was obtained from Nacalai (Kyoto, Japan). 5-Hydroxy-1,4-NQ (95% purity determined by HPLC), NAPQI, BPM, and the HRP-linked anti-biotin antibody were purchased from Sigma-Aldrich (St. Louis, MO, USA), Invitrogen (Carlsbad, CA, USA) and Cell Signaling Technology (Beverly, MA, USA), respectively. Vapor phase samples were collected in the mid-town area of Los Angeles near the University of Southern California during June (from 17 th to 22 th , VP1) and July (from 1 st to 7 th , VP2) 2008 and extracted as reported previously (Eiguren-Fernandez et al., 2008 , 2010) . All other reagents were of the highest grade available.

Abiko Y., Luong N.C, & Kumagai Y. (2015). A Biotin-PEAC5-maleimide labeling assay to detect electrophiles. The Journal of toxicological sciences, 40(3).

+ Open protocol

+ Expand

HEI-OC1 Cell Viability Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immortomouse^™-derived HEI-OC1 cells were grown in plastic cell culture dishes at permissive conditions, 33 °C, 5% CO2 in DMEM supplemented with 10% FBS, as previously described (Kalinec et al., 2003 (link)). HEI-OC1 cells were first trypsinized, counted and concentration adjusted to 5.0 × 10⁵ cells/mL using Cellometer Auto T4 (Nexelcom Bioscience, Lawrence, MA). Next, cells were transferred to 48-well flat bottom plates (250 μL each well), incubated again at 33°C for 4 h, and then some of them exposed to differing concentrations of APAP (0.3, 0.6, 1.5, 3.75, 7.5 mg/mL) and NAPQI (30, 60, 150, 375, 750 μg/mL) (Sigma-Aldrich, Saint Louis, MO) whereas others were not exposed to any drug (Control). Direct cell count was performed at different time points (0, 24, and 48 h) using Celigo, an adherent cell cytometer (Cyntellect Inc., San Diego, CA).

Kalinec G.M., Thein P., Parsa A., Yorgason J., Luxford W., Urrutia R, & Kalinec F. (2014). Acetaminophen and NAPQI are Toxic to Auditory Cells via Oxidative and Endoplasmic Reticulum Stress-dependent Pathways. Hearing research, 313, 26-37.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!