β ram in us detector

The HPLC (high performance liquid chromatography) method used an Agilent HP-1100 HPLC system (Agilent Technologies, Santa Clara, CA), equipped with a β-ram IN/US detector (Tampa, FL) and Zorbax C₈ column (4.6 mm×250 mm, 300 Å pore size; Agilent Technologies, Santa Clara, CA). The flow rate was 1 mL/min. The mobile phase was isocratic with 30% solvent A (10 mM NH₄OAc buffer, pH = 6.8) and 70% solvent B (methanol) between 0 and 5 min, followed by a gradient from 70% solvent B at 5 min to and 90% solvent B at 20 min. The RCP for ^99mTcN-MPO was calculated as the percentage of peak area over the total area. ITLC (instant thin layer chromatography) used Gelman Sciences silica-gel strips and a 1:1 mixture of acetone/saline (v:v) as eluent. ^99mTcN-MPO and ^99mTcO₄⁻ migrated to solvent front while [^99mTc]colloid stayed at the origin. [^99mTc]colloid was reported as the percentage of radioactivity at the origin over the total radioactivity on each strip.

The radio-HPLC method for routine analysis of ^99mTc(III)-azide complexes [^99mTc(N₃)(CDO)(CDOH)₂B-R] (R = IS, MP, PA, PY and 5U) used an Agilent HP-1100 HPLC system (Agilent Technologies, Santa Clara, CA) equipped with a β-ram IN/US detector (Tampa, FL) and Zorbax C₈ column (4.6 mm × 250 mm, 300 Å pore size; Agilent Technologies, Santa Clara, CA). The flow rate was 1 mL/min. The mobile phase was isocratic between 0 and 5 min with 30% solvent A (10 mM NH₄OAc buffer, pH = 6.8) and 70% solvent B (methanol), followed by a gradient from 70% solvent B at 5 min to and 90% solvent B at 20 min. The radiochemical purity (RCP) was reported as the percentage of area for the expected radiometric peak on each radio-HPLC chromatogram of each ^99mTc(III) radiotracer. The ITLC method used Gelman Sciences silica-gel strips and a 1:1 (v:v) mixture of acetone and saline as the mobile phase. ^99mTc radiotracers and ${{}^{99m}\text{TcO}}_4^{-}$ migrated to solvent front while [^99mTc]colloid stayed at the origin.