Fhs 74 int

Manufactured by American Type Culture Collection

Sourced in United States

The FHs 74 Int is a cell line derived from human fetal small intestine tissue. It serves as a model for the study of intestinal cell biology and function.

Automatically generated - may contain errors

Lab products found in correlation

18 protocols using fhs 74 int

Culturing Human Fetal Intestinal and Rat Intestinal Epithelial Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The non-transformed human fetal intestinal epithelial cell line FHs74Int and the IEC6 cell line were obtained from the American Type Culture Collection (ATCC). FHs74Int cells were cultured in Hybri-Care Medium (ATCC-46X) supplemented with 10% fetal bovine serum (FBS), 1% antibiotic-antimycotic and 30 ng/mL rhEGF. IEC6 cells were cultured in DMEM supplemented with 10% fetal bovine serum and 1% antibiotic-antimycotic. All cells were maintained at 37°C and 5% CO2.

Guo T., Hu S., Xu W., Zhou J., Chen F., Gao T., Qu W., Chen F., Lv Z, & Lu L. (2023). Elevated expression of histone deacetylase HDAC8 suppresses arginine-proline metabolism in necrotizing enterocolitis. iScience, 26(6), 106882.

+ Open protocol

+ Expand

Intestinal Cell Line Cultures

Check if the same lab product or an alternative is used in the 5 most similar protocols

One representative normal intestinal cell line (FHs 74 Int (ATCC CCL 241)) and two cancer intestinal cell lines (Caco-2 (ATCC HTB 37) and HT29 (ATCC HTB 38)) were purchased from ATCC (Rockville, MD, USA). Normal cells were cultured in Hybri-Care medium supplemented with 10% fetal bovine serum, 1% sodium bicarbonate, 1% non-essential amino acids, 30 ng/mL of epidermal growth factor, and 1% penicillin-streptomycin solution (10,000 units/mL and 100 mg/mL, respectively). The cancer cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 1% sodium pyruvate, 10% fetal bovine serum, 1% sodium bicarbonate, 1% non-essential amino acids, and 1% penicillin-streptomycin solution (10,000 units/mL and 100 mg/mL, respectively) (all purchased from Sigma-Aldrich, Prague, Czech Republic). The cultures were incubated at 37 °C and 5% CO₂. The culture medium was replaced every 2–3 days and cells were passaged every 7 days.

Kudera T., Doskocil I., Salmonova H., Petrtyl M., Skrivanova E, & Kokoska L. (2020). In Vitro Selective Growth-Inhibitory Activities of Phytochemicals, Synthetic Phytochemical Analogs, and Antibiotics against Diarrheagenic/Probiotic Bacteria and Cancer/Normal Intestinal Cells. Pharmaceuticals, 13(9), 233.

+ Open protocol

+ Expand

Culture of Intestinal Epithelial Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human colonic epithelial cells (FHC) and small intestinal epithelial cells (ATCC CCL-241; FHs74Int) (CRL-1831, ATCC, Manassas, VA) were cultured as described previously.^{10 (link)}

Zhou Q., Costinean S., Croce C.M., Brasier A.R., Merwat S., Larson S.A., Basra S, & Verne G.N. (2014). MicroRNA 29 Targets Nuclear Factor-κB–Repressing Factor and Claudin 1 to Increase Intestinal Permeability. Gastroenterology, 148(1), 158-169.e8.

+ Open protocol

+ Expand

Caco-2 Cell Culture and EV Isolation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Caco-2 HTB-37 (ATCC, USA),
FHs 74 Int (ATCC, USA), streptomycin/penicillin solution (Gibco, USA),
phosphate-buffered saline (PBS) (Gibco, USA), and trypsin/EDTA (Gibco,
USA), β-Cyclodextrin (Sigma Aldrich, USA), Dulbecco’s
modified eagle medium (ATCC, USA), fetal bovine serum (Gibco, USA),
and EV (Toronto Research Chemicals, Canada).

Maki M.A., Kumar P.V., Cheah S.C., Siew Wei Y., Al-Nema M., Bayazeid O, & Majeed A.B. (2019). Molecular Modeling-Based Delivery System Enhances Everolimus-Induced Apoptosis in Caco-2 Cells. ACS Omega, 4(5), 8767-8777.

+ Open protocol

+ Expand

Engineered Keratinocyte Delivery for Wound Healing

Check if the same lab product or an alternative is used in the 5 most similar protocols

Recombinant human keratinocyte growth factor (Sigma, USA), chitosan (Aldrich, Iceland), sodium tripolyphosphate (Sigma Aldrich, USA), trehalose (Merck, Germany), methacrylic acid-methyl methacrylate copolymer (1:1) 135,000 MW (Kollicoat MAE 100 P) (Sigma, Germany), KGF (FGF7) ELISA kit (abcam, UK), Rhodamine 6G (Sigma, Germany), Thiazolyl Blue Tetrazolium Bromide powder (MTT) (Sigma Aldrich, USA), DAPI (4',6-diamidino-2-phenylindole) (Sigma, Germany), FHs 74 Int (ATCC, USA), Hybri-Care Medium 46-X (ATCC, USA), GibcoTM Dulbecco’s Modified Eagle’s Medium (DMEM) (Thermo Fisher Scientific, USA) and veterinary capsules size 5 for rabbits (Torpac Inc., USA).

Kumar P.V., Maki M.A., Wei Y.S., Tatt L.M., Elumalai M., Cheah S.C., Raghavan B, & Majeed A.B. (2019). Rabbit as an Animal Model for Pharmacokinetics Studies of Enteric  Capsule Contains Recombinant Human Keratinocyte Growth Factor Loaded Chitosan Nanoparticles. Current Clinical Pharmacology, 14(2), 132-140.

+ Open protocol

+ Expand

Intestinal Cell Culture Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

One representative of normal intestinal cell line (FHs 74 Int [ATCC CCL 241]) and two of cancer intestinal cell lines (Caco-2 [ATCC HTB 37]) and HT-29 [ATCC HTB 38]) were purchased from ATCC (Rockville, MD, United States). Normal cells were cultured in Hybri-Care medium supplemented with 10% fetal bovine serum, 1% sodium bicarbonate, 1% nonessential amino acids, 30 ng/ml of epidermal growth factor, and 1% penicillin-streptomycin solution (10,000 units/ml and 100 mg/ml, respectively). The cancer cells were cultured in Eagle’s Minimum Essential Medium (EMEM) supplemented with 1% sodium pyruvate, 10% fetal bovine serum, 1% sodium bicarbonate, 1% nonessential amino acids, and 1% penicillin-streptomycin solution (10,000 units/ml and 100 mg/ml, respectively) (all purchased from Biowest, Nuaille, France). The cultures were incubated at 37°C and 5% CO₂. The culture medium was replaced every 2–3 days, and cells were passaged every 7 days.

Kudera T., Fiserova B., Korytakova M., Doskocil I., Salmonova H., Tulin E.E., Nguon S., Bande M.M, & Kokoska L. (2021). In Vitro Selective Antibacterial and Antiproliferative Effects of Ethanolic Extracts from Cambodian and Philippine Plants Used in Folk Medicine for Diarrhea Treatment. Frontiers in Pharmacology, 12, 746808.

+ Open protocol

+ Expand

Pancreatic Cancer Cell Line Cultivation

Cited 3 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

AsPC-1, HPAF-II, MIA-PaCa-2, BxPC3, Capan-2, and CFPAC-1 cells were obtained from ATCC (Manassas, Virginia) and authenticated (via short tandem repeat profiling). FHs-74Int cells were also obtained directly from ATCC. Mouse PDAC cells derived from a pancreatic tumor of a LSL-KRas^G12D, LSL-Trp53^−/−, PDX-1-CRE, (KPC) genetically engineered mouse model of PDAC and transfected with enhanced firefly luciferase (KPC-Luc) as previously described were provided by Dr. Cruz-Monserrate (17 (link), 18 (link)). Stable shRNA Cav-1 knockdown MIA-PaCa-2 cells were generated as described previously (16 (link)). Cells were maintained at 37°C in 5% CO₂ in RPMI 1640 media (AsPC-1), MEM (HPAF-II), DMEM (MIA-PaCa-2, G37, KPC-Luc), or ATCC Hybri-Care Medium 46-X (DMEM-like) + 30 ng/ml EGF (FHs 74Int) supplemented with 10% FBS and 1% penicillin/streptomycin. Cells were cultured for no more than 3 months continuously. Gemcitabine, nab-paclitaxel, trametinib, and RO-3306 were added to media with a vehicle final concentration of no more than 0.1%.

Wolfe A.R., Robb R., Hegazi A., Abushahin L., Yang L., Shyu D.L., Trevino J.G., Cruz-Monserrate Z., Jacob J., Palanichamy K., Chakravarti A, & Williams T.M. (2020). Altered Gemcitabine and Nab-paclitaxel Scheduling Improves Therapeutic Efficacy Compared to Standard Concurrent Treatment in Pre-clinical Models of Pancreatic Cancer. Clinical cancer research : an official journal of the American Association for Cancer Research, 27(2), 554-565.

+ Open protocol

+ Expand

Establishing Highly Invasive CRC Cell Line

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human colon cancer cell lines SW480, SW620, HCT116, RKO, and normal small intestine epithelial cell FHs 74Int were purchased from ATCC (Rockville, MD), and the CRC cells were maintained in 1640 medium supplemented with 10% fetal bovine serum (FBS, Gibco, CA, USA) at 37°C with 5% CO₂. HCT116 highly invasive cell line (HCT116-i8) was established using repeated invasion assays as previously described 21 (link). All the cell lines were tested for mycoplasma contamination. U0126 and trichostatin A (TSA) were purchased from Selleck Chemicals (cat#S1102 and cat#S1045, Houston, TX, USA). U0126 (10 mM, 10 mg in 2.35 mL dimethyl sulfoxide (DMSO)), and TSA (10 mM, 5 mg in 1.65 mL DMSO) were respectively dissolved for in vitro usage.

Huang Z.J., Li Y.J., Yang J., Huang L., Zhao Q., Lu Y.F., Hu Y., Zhang W.X., Liang J.Z., Pan J., Pan Y.L., He Q.Y, & Wang Y. (2024). PTPLAD1 Regulates PHB-Raf Interaction to Orchestrate Epithelial-Mesenchymal and Mitofusion-Fission Transitions in Colorectal Cancer. International Journal of Biological Sciences, 20(6), 2202-2218.

+ Open protocol

+ Expand

FHs 74 int Cell Response to LPS, Rottlerin, and Epac Inhibitor

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human intestinal epithelial cell line FHs 74 int (ATCC CCL-241) was purchased from the Beijing Institute of Oncology. The cells were cultured in Hybri Care medium (ATCC 46X) reconstituted following the instructions of the ATCC and contained 10% FBS (Sigma, Cat #: 12007C) and 45 ng/mL epidermal growth factor (Sigma, Cat #: E5160). For execution experiments, cells were inoculated in 6-well cell culture cluste (4 × 10 5 cells/well). Cells were cultured for 24 hours with (1) control (normal DMEM); (2) LPS treatment (10 µg/mL LPS) [21] ; (3) LPS with rottlerin (dissolved in DMSO, Sigma Aldrich, Cat #: D4540) treatment (10 µg/mL LPS and 1 μM rottlerin) [15] ; or (4) LPS with rottlerin and HJC0197 (Epac inhibitor, dissolved in DMSO, APExBIO, Cat #: 1383539-73-8) (10 µg/mL LPS, 1 μM rottlerin and 10 μM HJC0197) [22] .

Song X., Zuo L., Wang L., Zhu Z., Tao J., Jiang Y., Wu X., Wang Z., Nian J., Xiang P., Zhang Q., Zhao H., Liang Y., Li J., & Hu J. (2020). Rottlerin ameliorates DSS-induced colitis by improving intestinal barrier function via activation of the Epac-2/Rap-1 signaling pathway.

+ Open protocol

+ Expand

Optimizing Human Colon Cancer Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human colon cancer cells, SW480, SW620, HCT116, RKO, and FHs 74 Int (ATCC, Rockville, MD), were maintained in 1640 medium supplemented with 10% fetal bovine serum (Thermo Fisher Scienti c, Waltham, MA, USA) at 37°C with 5% CO 2 . The HCT116 highly invasive (HCT116 I8) cell line was screened by using repeated invasion assays as previously described [25] . All the cell lines were authenticated by short tandem repeat pro ling and tested for mycoplasma contamination. U0126, trichostatin A (TSA) and avobenzone were purchased from Selleck Chemicals (Houston, TX, USA). Respectively, U0126 (10mM, 10mg in 2.34mL dimethyl sulfoxide (DMSO)), TSA (10mM, 5mg in 1.65mL DMSO), and avobenzone (10mM, 10mg in 3.22mL DMSO) were dissolved for in vitro usage. Avobenzone (2mg/mL, 14mg) was dissolved in corn oil (7mL) for in vivo usage.

Yang J., Li Y., Hu Y., Zhang W., Xin Y., Liang J., Huang X., Li B., & He Q. (2021). Identification of PTPLAD1 as a Novel Tumor Suppressor and its Implication in Metastatic Colon Cancer Therapy.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!