Cyanine-3 (Cy3)-labeled cRNA was prepared from 0.5 μg input RNA using Agilent Technologies’ One-Color Quick Amp labeling kit according to the manufacturer’s instructions, followed by purification of cRNA by RNAeasy column (QIAGEN, Valencia, CA). Cy3-dye incorporation and yield of cRNA was checked with a NanoDrop ND-1000 spectrophotometer. The specific activity of all cRNA samples ranged from 10.98 to 20.02 (mean 14.94). cRNA (1.65 mg) was fragmented and hybridized to Agilent’s whole genome microarrays (G4112A) at 65°C for 17 hr with rotation, followed by washing according to the manufacturer’s recommendations. Microarray slides were scanned immediately with the Agilent scanner (G2404B) using the recommended settings and the images were analyzed with Feature Extraction 9.1 (Agilent Technologies) using default parameters.
Whole genome microarrays g4112a
Manufactured by Agilent Technologies
Whole genome microarrays (G4112A) are a laboratory instrument used for the analysis of gene expression. The core function of this product is to simultaneously measure the expression levels of thousands of genes within a biological sample.
Automatically generated - may contain errors
2 protocols using whole genome microarrays g4112a
1
Cyanine-3 RNA Labeling and Microarray
2
Cyanine-3 RNA Labeling and Microarray
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Cyanine-3 (Cy3)-labeled cRNA was prepared from 0.5 μg input RNA using Agilent Technologies’ One-Color Quick Amp labeling kit according to the manufacturer’s instructions, followed by purification of cRNA by RNAeasy column (QIAGEN, Valencia, CA). Cy3-dye incorporation and yield of cRNA was checked with a NanoDrop ND-1000 spectrophotometer. The specific activity of all cRNA samples ranged from 10.98 to 20.02 (mean 14.94). cRNA (1.65 mg) was fragmented and hybridized to Agilent’s whole genome microarrays (G4112A) at 65°C for 17 hr with rotation, followed by washing according to the manufacturer’s recommendations. Microarray slides were scanned immediately with the Agilent scanner (G2404B) using the recommended settings and the images were analyzed with Feature Extraction 9.1 (Agilent Technologies) using default parameters.
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