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2 protocols using rabbit polyclonal anti histone h3 antibody

1

Antibody Procurement for Cell Biology

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Rabbit polyclonal anti-Esco1 antibody was purchased from Santa Cruz Biotechnology (Dallas, TA, USA; Cat#:sc-135051); Rabbit polyclonal anti-Esco2 antibody was purchased from Bethyl Laboratories (Montgomery, TX, USA: Cat#: A301-689A-T); mouse monoclonal anti-α-tubulin-fluorescein isothiocyanate (FITC) antibody and mouse monoclonal anti-acetyl-α-tubulin (Lys 40) antibody were purchased from Sigma (St Louis, MO, USA; Cat#: F2168, T7451); human anti-centromere antibody was purchased from Antibodies Incorporated (Davis, CA, USA; Cat#: 15-234); Rabbit polyclonal anti-histone H3 antibody was purchased from Abcam (Cambridge, MA, USA; Cat#: ab1791); Rabbit monoclonal anti-Gapdh antibody was purchased from Cell Signaling Technology (Danvers, MA, USA; Cat#: 2118); HRP-conjugated Streptavidin antibody was purchased from Jackson ImmunoResearch Laboratories (West Grove, PA, USA; Cat#: 016-030-084); FITC-conjugated goat anti-rabbit IgG (H + L), TRITC-conjugated goat anti-rabbit IgG (H + L) and FITC-conjugated goat anti-mouse IgG (H + L) were purchased from Zhongshan Golden Bridge Biotechnology Co., LTD (Beijing, China).
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2

Antibody Characterization for Nucleolar Proteins

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Mouse monoclonal anti-nucleolin antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA, C23 (MS-3), sc-8031), mouse monoclonal anti-β-actin antibody (Sigma, AC-15), mouse monoclonal anti-Flag antibody (ANTI-FLAG M2 Monoclonal Antibody, Sigma, F1804), mouse monoclonal anti-HP1γ antibody (Sigma, MAB3450), mouse monoclonal anti-RNA polymerase I antibody (Santa Cruz Biotechnology, anti-RPA194 antibody (C-1), sc-48385), rabbit polyclonal anti-GFP antibody (Santa Cruz, sc-8334), goat anti-rabbit IgG-HRP (Santa Cruz, sc-2054), goat anti-mouse IgG-HRP (Santa Cruz #sc-2005), Alexa 568-conjugated goat anti-mouse IgG (H+L) (Thermo Fisher Scientific, Waltham, MA, USA, A11004), Alexa 488-conjugated goat anti-mouse IgG (H+L) (Thermo Fisher, A11029), and Alexa 568-conjugated goat anti-rabbit IgG (H+L) (Thermo Fisher, A11036), mouse monoclonal anti-dimethylated histone H3 lys36 antibody (Active Motif, California, USA, MABI0332), and rabbit polyclonal anti-histone H3 antibody (Abcam, Cambridge, UK, ab1791), were purchased. The anti-KDM2A antibody was described previously [19 (link)]. The anti-Fbxl11 (KDM2A) antibody (Abcam, ab99242) was purchased and used to detect the C-terminal half of KDM2A.
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