Xk 16 60 superdex 75 column

Manufactured by GE Healthcare

Sourced in Brazil

The XK 16/60 Superdex 75 column is a size exclusion chromatography column designed for the separation and purification of proteins, peptides, and other biomolecules. The column features a Superdex 75 resin, which provides high-resolution separation over a wide range of molecular weights. The column dimensions are 16 mm in diameter and 60 cm in length.

Automatically generated - may contain errors

Lab products found in correlation

Ni sepharose resin, by GE Healthcare (3 mentions)

3 protocols using xk 16 60 superdex 75 column

Recombinant ZIKV NS5 RdRp Purification

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

ZIKV NS5 RdRp polymerase was cloned at pETTRX by the LIC method and expressed and purified according to the protocol described in [92 (link)]. Briefly, NS5 RdRp polymerase was expressed in ZYM 5052 auto-induction medium and purified in four steps: (i) a HisTrap HP 5.0 mL with a Ni Sepharose resin (GE Healthcare, Sao Carlos, Brazil); (ii) a buffer exchanged by dialysis and a concomitant TEV protease cleavage from 6His-TRX-tag; (iii) an inverse HisTrap HP 5.0 mL to separate protein from 6His-TRX-tag and (iv) a size-exclusion chromatography at a XK 16/60 Superdex 75 column (GE Healthcare, Sao Carlos, Brazil).

Ramos P.R., Mottin M., Lima C.S., Assis L.R., de Oliveira K.Z., Mesquita N.C., Cassani N.M., Santos I.A., Borba J.V., Fiaia Costa V.A., Neves B.J., Guido R.V., Oliva G., Jardim A.C., Regasini L.O, & Andrade C.H. (2022). Natural Compounds as Non-Nucleoside Inhibitors of Zika Virus Polymerase through Integration of In Silico and In Vitro Approaches. Pharmaceuticals, 15(12), 1493.

+ Open protocol

+ Expand

Purification of Dengue Virus Protease

Check if the same lab product or an alternative is used in the 5 most similar protocols

Each portion of cells was thawed in water before lysed by sonication. Protein extract was separated from cell debris by centrifugation. NS2B-NS3p purification was done in four steps: (i) a HisTrap HP 5.0 mL with a Ni Sepharose resin (GE Healthcare) pre-equilibrated with Buffer 1, where the protein was eluted by Buffer 1 supplemented with 500mM Imidazole; (ii) a buffer exchanged by dialysis to Buffer 1 and a concomitantly TEV protease cleavage from 6His-SUMO-tag during 16; (iii) an inverse HisTrap HP 5.0 mL to separate NS2B-NS3p from 6His-SUMO-tag; and (iv) a size-exclusion chromatography at a XK 16/60 Superdex 75 column (GE Healthcare) in buffer 20 mM Hepes, pH 8.0, 500 mM NaCl, 5% glycerol.

Lima C.S., Mottin M., de Assis L.R., de Moraes Roso Mesquita N.C., de Paula Sousa B.K., Coimbra L.D., dos Santos K.B., Zorn K.M., Guido R.V., Ekins S., Marques R.E., Proença-Modena J.L., Oliva G., Andrade C.H, & Regasini L.O. (2021). Flavonoids from Pterogyne nitens as Zika virus NS2B-NS3 protease inhibitors. Bioorganic chemistry, 109, 104719.

+ Open protocol

+ Expand

Purification of Viral Replication Enzymes

Cited 3 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

NS5 polymerase, NS2B-NS3 protease, and NS3 helicase were cloned at pETTRX- or pETSUMO by LIC method, expressed and purified according to the protocols described by Silva and coworkers (2019)^{91 (link)}, Lima and coworkers (2021)^{92 (link)} and Godoy and coworkers (2017)^{93 (link)}. Briefly, the proteins were expressed in ZYM 5052 auto-induction medium or Luria Bertani (LB) medium and purified in four steps: (i) a HisTrap HP 5.0 mL with a Ni Sepharose resin (GE Healthcare); (ii) a buffer exchanged by dialysis and a concomitantly TEV protease cleavage from 6His-TRX-tag or 6His-SUMO-tag; (iii) an inverse HisTrap HP 5.0 mL to separate protein from 6His-TRX-tag and 6His-SUMO-tag; and (iv) a size-exclusion chromatography at an XK 16/60 Superdex 75 column (GE Healthcare).

Mottin M., de Paula Sousa B.K., de Moraes Roso Mesquita N.C., de Oliveira K.I., Noske G.D., Sartori G.R., de Oliveira Albuquerque A., Urbina F., Puhl A.C., Moreira-Filho J.T., Souza G.E., Guido R.V., Muratov E., Neves B.J., da Silva J.H., Clark A.E., Siqueira-Neto J.L., Perryman A.L., Oliva G., Ekins S, & Andrade C.H. (2022). Discovery of new Zika protease and polymerase inhibitors through the open science collaboration project OpenZika. Journal of chemical information and modeling, 62(24), 6825-6843.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!