Whole blood was lysed and labeled with a combination of the following monoclonal antibodies: MHC I-PE-Cy7 (G46-2.6, BD Biosciences, San Jose, CA), CD3-PerCP-Cy5.5 (SP34-2, BD Biosciences), CD4-BV510 (L200, BD Horizon, Franklin Lakes, NJ), CD4-APC (L200, BD Pharmingen, Franklin Lakes, NJ), CD8-APC (RPA-T8, BD Biosciences), CD8-BV421 (RPA-T8, BD Biosciences), CD95-PE (DX2, BD Biosciences), CD28-Pacific Blue (CD28.2, BioLegend, San Diego, CA). Samples were acquired using FACSCanto II or LSRFortessa (BD Bioscience). Samples were analyzed using FCS Express (De Novo Software, Glendale, CA). CD4+ and CD8+ T cells were defined as CD20-CD3+CD4+ or CD20-CD3+CD8+ cells, respectively. NK cells were defined as CD20-CD3-CD56+CD8+ cells.13 (link),14 (link) Naïve T cells were defined as CD28+CD95-. Central and effector memory T cells were defined as CD28+CD95+ and CD28-CD95+, respectively.
Cd95 pe dx2
Manufactured by BD
Sourced in United Kingdom
CD95-PE (DX2) is a fluorescently labeled antibody that binds to the CD95 (Fas) receptor. CD95 is a cell surface receptor that plays a role in regulating apoptosis. The PE (phycoerythrin) fluorescent label allows for the detection and analysis of CD95-expressing cells using flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
Fcs express, by De Novo Software (1 mentions)
Cd4 apc l200, by BD (1 mentions)
Cd8 apc rpa t8, by BD (1 mentions)
Cd4 bv510, by BD (1 mentions)
Facscanto 2, by BD (1 mentions)
Lsrfortessa, by BD (1 mentions)
Cd27 pc7 clone 1a4cd27, by Beckman Coulter (1 mentions)
Cd4 fitc clone 13b8.2, by Beckman Coulter (1 mentions)
Cd8 alexa fluor 700, by Beckman Coulter (1 mentions)
Kaluza software, by Beckman Coulter (1 mentions)
2 protocols using cd95 pe dx2
1
T cell and NK cell immunophenotyping
2
Comprehensive Immune Profiling of Surgical Patients
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Fasting venous blood (2 mL) was collected from participants in the study and control groups before surgery, and flow cytometry was performed within 24 h. Nucleated cells were stained with the following antibodies: CD3-Alexa Fluor 750 (clone UCHT1; Beckman Coulter, USA), CD4-FITC (clone 13B8.2; Beckman Coulter), CD8-Alexa Fluor 700 (clone B9.11; Beckman Coulter), CD28-PC5.5 (clone CD28.2; Beckman Coulter), CD27-PC7 (clone 1A4CD27; Beckman Coulter), CD45-Kro (clone J.33; Beckman Coulter), CD45RO-ECD (clone UCHL1; Beckman Coulter), CD95-PE (DX2; BD Biosciences, UK), and CCR7-APC (G043H7; BioLegend). The ten-color flow cytometer (Naivos; Beckman Coulter) was used for detection. The test results were analyzed using the Kaluza software (version 2.0, Beckman Coulter). The analysis scheme is illustrated in Figure 1
Carcinoembryonic antigen (CEA) and carbohydrate antigen 199 (CA199) were detected by an electrochemiluminescence immunoassay (ECLIA). The Roche Cobas e602 electrochemiluminescence immunoassay analyzer and its supporting reagents were used.
Carcinoembryonic antigen (CEA) and carbohydrate antigen 199 (CA199) were detected by an electrochemiluminescence immunoassay (ECLIA). The Roche Cobas e602 electrochemiluminescence immunoassay analyzer and its supporting reagents were used.
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