Antifoam ex cell

Manufactured by Merck Group

Antifoam EX-Cell is a laboratory product manufactured by Merck Group. It is designed to suppress foam formation in various laboratory processes. The core function of this product is to help control and manage foaming, which can be a common challenge in laboratory environments.

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Lab products found in correlation

Antifoam 204, by Merck Group (2 mentions) Antifoam c, by Merck Group (2 mentions) Opti cho medium, by Thermo Fisher Scientific (2 mentions) Cd dynamis, by Thermo Fisher Scientific (2 mentions) Powercho 2 cd, by Lonza (2 mentions) Antifoam se 15, by Merck Group (2 mentions) Procho5, by Lonza (2 mentions) Antifoam y 30, by Merck Group (2 mentions) Penicillin streptomycin, by Corning (2 mentions) L glutamine, by Corning (2 mentions) 1m naoh, by Thermo Fisher Scientific (1 mentions)

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Antifoam (12 citations)

2 protocols using antifoam ex cell

Optimizing CHO Cell Culture Media and Antifoams

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Chemically defined (CD) OptiCHO medium (Thermo Fisher Scientific, Waltham, MA) was the original media in which the cell line was propagated. The cells were adapted to four other commercially available media, including CD Dynamis (Thermo Fisher Scientific, Carlsbad, CA), protein‐free ProCHO 5 (Lonza, Walkersville, MD), CD PowerCHO 2 (Lonza, Walkersville, MD), and CD EX‐Cell Advanced (SAFC, Lenexa, KS). All media studied were supplemented with 8 mM L‐glutamine (Corning, Manassas, VA) and 1X Penicillin/Streptomycin (Corning, Oneonta, NY). Initial glucose concentration in each media varied, based on the manufacturer's proprietary formulation, and ranged between 5 and 8 g/L. ProCHO 5 and EX‐Cell Advanced had the highest glucose concentration, while OptiCHO had the lowest glucose concentration. Five commercially available antifoams (Sigma Life Sciences, St. Louis, MO) were studied: Antifoam C, Antifoam EX‐Cell, Antifoam 204, Antifoam SE‐15, and Antifoam Y‐30. Cell culture pH was controlled using 1M NaOH (Thermo Fisher Scientific, Fair Lawn, NJ).

Velugula‐Yellela S.R., Williams A., Trunfio N., Hsu C., Chavez B., Yoon S, & Agarabi C. (2017). Impact of media and antifoam selection on monoclonal antibody production and quality using a high throughput micro‐bioreactor system. Biotechnology Progress, 34(1), 262-270.

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Optimizing CHO Cell Culture Media

Check if the same lab product or an alternative is used in the 5 most similar protocols

Chemically-defined (CD) OptiCHO medium (Thermo Fisher Scientific, Waltham, MA) was the original media in which the cell line was propagated. The cells were adapted to four other commercially available media including: CD Dynamis (Thermo Fisher Scientific, Carlsbad, CA), protein-free ProCHO 5 (Lonza, Walkersville, MD), CD PowerCHO 2 (Lonza, Walkersville, MD), and CD EX-Cell Advanced (SAFC, Lenexa, KS). All media studied were supplemented with 8 mM L-glutamine (Corning, Manassas, VA) and 1X Penicillin/Streptomycin (Corning, Oneonta, NY). Initial glucose concentration in each media varied, based on the manufacturer’s proprietary formulation, and ranged between 5 and 8 g/L. ProCHO 5 and EX-Cell Advanced had the highest glucose concentration, while OptiCHO had the lowest glucose concentration. Five commercially available antifoams (Sigma Life Sciences, St. Louis, MO) were studied: Antifoam C, Antifoam EX-Cell, Antifoam 204, Antifoam SE-15 and Antifoam Y-30. Cell culture pH was controlled using 1M NaOH (Thermo Fisher Scientific, Fair Lawn, NJ).

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