Total proteins were extracted from mycelia cultured in liquid CM for 48 h as previously described (Li et al., 2011 (link)). Proteins were separated with 10% SDS‐PAGE and then transferred to polyvinylidene fluoride (PDVF) membranes. The target proteins on the PDVF membrane were detected with anti‐GFP and anti‐RFP antibodies (Abbkine), along with horseradish peroxidase (HRP)‐conjugated secondary antibodies (Abbkine). Signals were visualized with a Western Blotting Detection Kit (Advansta) and the membrane was photographed using the ChemiDoc Touch imaging system (Bio‐Rad). The protein loading was monitored by staining proteins in acrylamide gels with Coomassie Brilliant blue (CBB) for 2 h and destaining the gel overnight before imaging (Wang et al., 2018 (link)).
Horseradish peroxidase hrp conjugated secondary antibodies
Manufactured by Abbkine
Sourced in China
Horseradish peroxidase (HRP)-conjugated secondary antibodies are laboratory reagents used in various immunoassay techniques. They function as a detection tool, binding to primary antibodies and catalyzing a colorimetric or chemiluminescent reaction to visualize target proteins or molecules.
Automatically generated - may contain errors
Lab products found in correlation
Western blotting detection kit, by Advansta (1 mentions)
Chemidoc touch imaging system, by Bio-Rad (1 mentions)
Pparγ antibody, by Abcam (1 mentions)
Gapdh monoclonal antibody, by Kangchen Biotech (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Stat3, by Abcam (1 mentions)
P stat3, by Abcam (1 mentions)
2 protocols using horseradish peroxidase hrp conjugated secondary antibodies
1
Western Blot Analysis of Recombinant Proteins
2
PPARγ Competitive Binding Assay Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
GVS-12 (C20H16F3NO3, MW: 375.346, purity ≥ 98%) was designed and synthesized by our team (Fig. 1A ); LanthaScreen™ time-resolved fluorescence resonance energy transfer (TR-FRET) PPARγ competitive binding assay was purchased from Thermo Fisher Co., Ltd. (Thermo Fisher, MA, USA); fetal bovine serum (FBS) was obtained from PAA (Linz, Germany). TRIzol reagent was obtained from Invitrogen (Carlsbad, CA, USA). Rosiglitazone (a PPARγ agonist) was obtained from Sigma Co., Ltd. (St. Louis, MO, USA). STAT3, p-STAT3, suppressor of cytokine signaling 3 (SOCS3) and PPARγ antibodies were purchased from Abcam (Cambridge, UK); GAPDH monoclonal antibody was purchased from KangChen Bio-tech (Shanghai, China); horse radish peroxidase (HRP)-conjugated secondary antibodies were purchased from Abbkine (Redlands, USA). Other analytical reagent grade chemicals were obtained from Sinopharm Chemical Reagent Co., Ltd. (Nanjing, China).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!