Male c57bl 6 mice

Seven-week-old C57BL/6 male mice were purchased from Samtako (South Korea). Mice were maintained under standard conditions (24 ± 2 °C, 12 h day-night cycle, 50 ± 5% humidity) in a pathogen-free environment. Mice were allowed free access to food pellets and water throughout the study period. Experimental procedures and animal management procedures were approved by the Institutional Animal Care and Use Committee (IACUC) of Jeonbuk National University in Korea (approval number: NON2023-111). All methods were conducted following IACUC ethical guidelines and regulations supported by the Korean Council on Animal Care and Korean Animal Protection Law, 2007; Article 13 (Experiments with animals). All animal trials followed the ARRIVE 2.0 guidelines, including study design, animal numbers, randomization, and statistical methodologies.
Twenty-four WT mice were divided into 4 groups: the PBS group (n = 5), the amlexanox-only control group (n = 5), LPS positive control (n = 7), and the amlexanox plus LPS group (n = 7).WT mice were injected with amlexanox (50 mg/kg-i.p) or PBS as a control daily for 3 days and thereafter, injected with LPS (10 mg/kg) (Escherichia coli O111:B4 was obtained from Sigma-Aldrich, St. Louis, MO, USA) for 24 h. Figure 1A depicts the drug treatment schedule.

C57BL/6 male mice, 6 weeks old, body weight (BW) 22 ± 2 g were purchased from Samtako Inc. (Osan, Republic of Korea). The mice were housed under a humidity of 50 ± 5%, a temperature of 23 ± 1 °C, a 12 h/12 h light and dark cycle, and were provided free access to a standard diet and tap water. All mice were provided with proper care following the guidelines of the Institutional Animal Care and Use Committee (IACUC) of the Korea Institute of Oriental Medicine (KIOM; Daegu, Republic of Korea), who reviewed and approved this study (IACUC-KIOM-D-18-013).