Anti fcεriα clone mar 1

BMMCs were generated from BM cells of C57BL/6 mice (Japan SLC, Hamamatsu, Japan) by cultivation under IL-3-supplemented condition as previously described [34 (link)]. Peritoneal MCs were prepared from peritoneal exudate cells of mice as previously reported protocol with some modification [35 (link)]. Briefly, peritoneal cells were cultivated in the presence of SCF (10 ng/mL) and IL-3 (10 ng/mL) for 2 weeks at 37 °C in a humidified atmosphere in the presence of 5% CO₂. More than 95% of nonadherent cells were identified to be MCs by positive staining with anti-FcεRIα (clone MAR-1, BioLegend, San Diego, CA, USA) and anti-CD117 (clone 2B8, BioLegened) in flow cytometry analysis. Independent experiments were performed on different days with different preparations of BMMCs generated from different mice. All experiments using mice were performed following the guidelines from the Institutional Review Board at Tokyo University of Science, and the present study was approved by the Animal Care and Use Committees at Tokyo University of Science: K22005, K21004, K20005, K19006.