Animal blood counter abc vet

Manufactured by Horiba

Sourced in France

The Animal Blood Counter-ABC vet is a laboratory equipment designed to analyze and count various blood parameters in animal samples. It provides accurate and reliable measurements of red blood cells, white blood cells, and platelet counts, which are essential for veterinary diagnostic purposes.

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Lab products found in correlation

Rm2125, by Leica (1 mentions) Cyclophosphamide, by Merck Group (1 mentions) Cobas mira plus, by Roche (1 mentions) Triton x 100, by Merck Group (1 mentions) 5 fluorouracil 5 fu, by Merck Group (1 mentions) Tween 80, by Merck Group (1 mentions) Eno 20 nox analyzer, by Eicom (1 mentions)

Spelling variants of this product

ABC Vet (28 citations) Animal Blood Counter (6 citations) Scil Vet ABC animal blood counter (2 citations)

8 protocols using animal blood counter abc vet

Hematological Analysis of Heparinized Blood

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The heparinized blood samples were analyzed regarding number of Red Blood Cells (RBCs), White Blood Cells (WBCs), Hemoglobin concentration (Hb), Hematocrit (HCT), and differential count of polymorphs and lymphocytes according to standard methods using an Animal Blood Counter-ABC vet (Horiba ABX, France)³⁸ ^,³⁹ (38, 39)³⁷ ^,³⁸ (38, 39).

Mokhtari T., Hussein Osman H.E., El-Meghawry El-Kenawy A, & Dashti N. (2019). Ameliorative effect of virgin olive oil against nephrotoxicity following sub-chronic administration of ethephon in male rats. Journal of Traditional and Complementary Medicine, 10(5), 487-495.

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Comprehensive Assessment of Biological Parameters

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Blood and tissue samples from groups A, G &H were assessed for biochemical, hematological and histopathological changes. Biochemical profiles of heart, kidney, and liver functions were assessed by measuring the serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), albumin, bilirubin, total protein, creatine kinase (CK-MB), lactate dehydrogenase (LDH), creatinine and blood urea. All tests were performed according to the standard methods (Scott et al., 2012 (link)). Hematological parameters; hemoglobin, hematocrit (HCT), red blood cells (RBC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), white blood cell (WBC), granulocyte count and lymphocytes were determined using Animal Blood Counter-ABC vet (Horiba ABX, France).
For histopathological studies, at autopsy, one gram from liver, heart, lung, kidney, and spleen was fixed in 10% formaldehyde; dehydrated through ascending grades of alcohol, cleared in xylene, wax-impregnated and embedded in paraffin wax. They were sectioned using microtome (Leica RM 2125, Leica Biosystems Nussloch GmbH, Germany) and stained with haematoxylin and Eosin and examined for histopathological changes using light microscope (Olympus BH-2, Olympus, Tokyo, Japan) (Fowotade et al., 2017 (link)).

Farrag H.A., Hosny A.E., Hawas A.M., Hagras S.A, & Helmy O.M. (2019). Potential efficacy of garlic lock therapy in combating biofilm and catheter-associated infections; experimental studies on an animal model with focus on toxicological aspects. Saudi Pharmaceutical Journal : SPJ, 27(6), 830-840.

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Comprehensive Blood Analysis Protocol

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The heparinized blood samples were analyzed for the number of red blood cells (RBCs), white blood cells (WBCs), and platelets, hemoglobin concentration (Hb%), hematocrit value (HTC), packed cells volume (PCV), mean corpuscle volume (MCV), mean corpuscle hemoglobin (MCH), mean corpuscle hemoglobin concentration (MCHC) and the differential count of polymorphs and lymphocytes according to standard methods using an Animal Blood Counter-ABC vet (Horiba ABX, France).

, & Nasr A.Y. (2014). Protective effect of aged garlic extract against the oxidative stress induced by cisplatin on blood cells parameters and hepatic antioxidant enzymes in rats. Toxicology Reports, 1, 682-691.

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Analytical techniques for 5-FU study

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5-Fluorouracil (5-FU), Triton X-100, Tween 80, and cyclophosphamide were purchased from Sigma-Aldrich (St. Louis, MO, USA). Dimethylsulfoxide (DMSO) was purchased from Mallinckrodt Chemicals (Phillipsburg, NJ, USA). Kits for biochemical analysis were purchased from Labtest (Lagoa Santa, MG, Brazil). An automatic biochemical analyzer (COBAS Mira Plus, Roche Diagnostic Systems) and an automatic hematological analyzer (Animal Blood Counter -ABC Vet, Horiba) were used.

Xavier A.L., Pita J.C., Brito M.T., Meireles D.R., Tavares J.F., Silva M.S., Maia J.G., Andrade E.H., Diniz M.F., Silva T.G., Pessoa H.L, & Sobral M.V. (2015). Chemical composition, antitumor activity, and toxicity of essential oil from the leaves of Lippia microphylla. Zeitschrift fur Naturforschung. C, Journal of biosciences, 70(5-6).

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Comprehensive Hematological and Biochemical Analysis

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After blood samples were collected into EDTA tubes, the following hematological parameters were analyzed using a Vet abc™ Animal Blood Counter (Horiba ABX, Montpellier, France): white blood cell (WBC), red blood cell (RBC), hemoglobin (HGB), hematocrit (HCT), platelet (PLT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), and differential WBC (neutrophils, eosinophils, basophils, lymphocytes, and monocytes). The following serum biochemistry parameters were also measured using serum immediately separated from whole blood samples collected into tubes without anticoagulant with an automatic chemistry analyzer (Hitachi 7070, Hitachi, Tokyo, Japan): blood urea nitrogen (BUN), total cholesterol (TC), total bilirubin (TB), total protein (TP), albumin, alkaline phosphatase (ALP), aspartate transaminase (AST), alanine transaminase (ALT), creatinine, and triglyceride (TG).

Yun J.W., Kwon E., Kim Y.S., Kim S.H., You J.R., Kim H.C., Park J.S., Che J.H., Lee S.K., Jang J.J., Kim H.H, & Kang B.C. (2018). Assessment of acute, 14-day, and 13-week repeated oral dose toxicity of Tiglium seed extract in rats. BMC Complementary and Alternative Medicine, 18, 251.

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Blood Count and Nitric Oxide Quantification

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Blood was collected in citrate (1:10). Blood count was quantified with a Vet abc animal blood counter (Horiba Medical; Grabels, France), while plasma NO₂⁻ and NO₃⁻ concentrations were measured with an ENO-20 NOx Analyzer (Eicom Corp., Kyoto, Japan).

Smeda M., Jasztal A., Maleki E.H., Bar A., Sternak M., Kwiatkowski G., Suraj-Prażmowska J., Proniewski B., Kieronska-Rudek A., Wojnar-Lason K., Skrzypek K., Majka M., Chrabaszcz K., Malek K, & Chlopicki S. (2022). Endothelial-mesenchymal transition induced by metastatic 4T1 breast cancer cells in pulmonary endothelium in aged mice. Frontiers in Molecular Biosciences, 9, 1050112.

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Whole Blood Hematology Analysis

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Whole blood samples were analyzed with an automatic Vet abc™ Animal Blood Counter (Horiba ABX, Montpellier, France) using the hematology kits specified for that instrument (Horiba ABX, France) according to the manufacturer’s instructions.

Altoom N.G., Ajarem J., Allam A.A., Maodaa S.N, & Abdel-Maksoud M.A. (2017). Deleterious effects of potassium bromate administration on renal and hepatic tissues of Swiss mice. Saudi Journal of Biological Sciences, 25(2), 278-284.

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Isoflurane Anesthesia and Blood Analysis

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After six weeks, the mice were subjected to anesthesia by putting them in an anesthesia drop-chamber. Inside the chamber, a cotton gauze that was previously soaked with 2 mL of 20% isoflurane in propylene glycol was present. After that, cervical dislocation was done, and blood samples were collected from all animals, and plasma was separated. For cell blood count (CBC), heparinized blood samples were analyzed using Vet abc™ Animal Blood Counter (Horiba ABX, Montpellier, France). For glucose, cholesterol, triglycerides, and low-density lipoprotein (LDL) estimation; Reflotron^®® plus was utilized using 30-µL plasma samples being put in specific device strips.

Al-Qahtani A., Ajarem J., Okla M.K., Rubnawaz S., Alamri S.A., Al-Qahtani W.H., Al-Himaidi A.R., Elgawad H.A., Akhtar N., Maodaa S.N, & Abdel-Maksoud M.A. (2022). Protective Effects of Green Tea Supplementation against Lead-Induced Neurotoxicity in Mice. Molecules, 27(3), 993.

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