TNBC cells were seeded on the sterile cover glass and stained with mitoSOXTM Red (M36008, Thermo Scientific), according to the manufacturing instructions. Cells were then fixed with 4% paraformaldehyde (158127, Sigma) for 10 min, permeabilized with DPBS containing 0.2% Triton X-100 (T8787, Sigma) for 5 min at room temperature, and co-stained with DAPI (300 nM, D3571, NEB). Cells were mounted using Fluoromount-G (0100-01, SouthernBiotech) on glass slides (12-550-15, Fisher Scientific). Images were captured using an RVL-100-G microscope (ECHO).
Rvl 100 g microscope
Manufactured by Echo Medical Systems
The RVL-100-G is a high-performance microscope designed for laboratory applications. It features a 40x-1000x magnification range, a built-in LED illumination system, and a sturdy, ergonomic design. The RVL-100-G is capable of delivering clear, detailed images for a variety of sample types.
Automatically generated - may contain errors
2 protocols using rvl 100 g microscope
1
Mitochondrial ROS Detection in TNBC
2
Liver and Adipose Tissue Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Liver and adipose tissue samples were dissected and rinsed in PBS (Beyotime, #ST448) before subsequent overnight fixation in 4% paraformaldehyde (Beyotime, #P0099). The paraffin embedding, sectioning, and H&E staining were conducted by Wuhan Servicebio Technology Co., Ltd (Wuhan, Hubei, China). Images (bright-field and fluorescence) were captured using an ECHO RVL-100-G microscope. Analysis of adipocyte size utilized bright-field H&E staining images, and the quantification was performed with ImageJ software.
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