Ab56918

After paraffin embedding, samples were prepared in 4-μm slices, followed by dehydration and dewaxing overnight in a drying oven at 65 °C and then completely dewaxed in xylene and gradient alcohol solutions. After high-pressure antigen repair with EDTA alkaline or sodium citrate repair solution, the sections were subjected to 12-min incubation with 30% hydrogen peroxide, and another 30-min incubation using goat serum. Then, antibodies against ROD1 (1:500, # ab56918, Abcam, UK), YTHDC1 (1:500, # ab259990, Abcam, UK), OIP5 (1:300, # TD12217S, Abmart, China), GPD1L (1:200, # PS18323S, Abmart, China), N-cadherin (1:400, # bs1172R, Bioss, China), E-cadherin (1:500, # ab40772, Abcam, UK), and vimentin (1:400, # bs0756R, Bioss, China) were supplemented for overnight incubation under 4 °C. The next day, we introduced the secondary antibody for 1-h incubation, DAB color developing solution was supplemented, and hematoxylin was used to restain the nucleus.

The proteins were transferred to NC membranes (Beyotime, China), and the membranes were incubated with primary antibodies against ROD1 (1:1000, # ab56918, Abcam, UK), OIP5 (1:1000, # TD12217S, Abmart, China), YTHDC1 (1:1000, # ab259990, Abcam, UK) and GPD1L (1:1000, # PS18323S, Abmart, China) at 4 °C overnight, followed by incubation with goat anti-rabbit secondary antibodies (1:2500, # EK020, ZZBIO, China) for 1 h. Then, antibody binding was identified with an ECL Ultrasensitive Chemiluminescent Solution (AccuRef Scientific, China).