Total RNA (Wild type (WT, control) 90: n = 6; SOD1 90: n = 6; RAGE KO SOD1 90: n = 5)and protein (WT 90 and 150: n = 3; SOD1 90 and 150: n = 3; RAGE KO SOD1 90 and 150: n = 4) from the same lumbar spinal cord neuromere in each animal studied was isolated using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol. The quantity and quality of RNA was verified spectrophotometrically using Thermo ScientificTM NanoDrop TM 2000/2000c Spectrophotometer (Thermo Fisher Scientific, Waltham, Massachusetts, USA). The protein concentration was determined using Direct Detect® Infrared Spectrometer for Total Protein Quantitation (Merck Millipore, Darmstadt, Germany).
Thermo scientific nanodrop tm 2000 2000c spectrophotometer
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Thermo Scientific™ NanoDrop™ 2000/2000c Spectrophotometer is a compact, high-performance instrument designed for the analysis of small-volume samples. It utilizes a patented sample-retention technology to enable measurements of volumes as low as 0.5 μL, with a wide linear range and high accuracy. The instrument is capable of measuring the absorbance of various biomolecules, including nucleic acids and proteins, across a wavelength range of 190 to 840 nm.
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2 protocols using thermo scientific nanodrop tm 2000 2000c spectrophotometer
1
RNA and Protein Isolation from Spinal Cord
2
RNA and Protein Isolation from Spinal Cord
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Total RNA and protein from the same lumbar spinal cord neuromere in each animal studied were isolated using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol. The quantity and quality of RNA were verified spectrophotometrically using Thermo ScientificTM NanoDrop TM 2000/2000c Spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). The protein concentration was determined using Direct Detect® Infrared Spectrometer for Total Protein Quantitation (Merck Millipore, Darmstadt, Germany).
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