The collected samples (nasopharyngeal swabs and lung tissues) were inoculated in Casein/sucrose/yeast broth (Oxoid) for 6–8 h. A loopful was cultivated on Casein/sucrose/yeast agar (CSY), blood agar and MacConkey agar plates then incubated at 37 °C for 48 h. The suspected colonies with culture characters of P. multocida were identified traditionally according to Quinn et al. (2002) using API 20 E tests (BioMérieu).
Casein sucrose yeast broth
Manufactured by Thermo Fisher Scientific
Casein/sucrose/yeast broth is a culture medium used for the growth and cultivation of microorganisms in laboratory settings. It provides a source of nitrogen, carbohydrates, and nutrients to support the growth of a variety of bacterial and fungal species.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using casein sucrose yeast broth
1
Isolation and Identification of Pasteurella multocida
2
Bacterial Identification Protocol for P. multocida
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All the collected samples were cultured primary into casein sucrose yeast broth (Oxoid) for 6–8 h and a loopful from each broth were sub-cultured on to blood agar and MacConkey agar media and incubated for 48 h at 37 °C. The recovered strains of P. multocida were completely identified with API 20 E tests (BioMérieu) according to Jakeen et al. (2016) (link).
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