NOD (#001976; Jax), BDC2.5 (#004460; Jax), NOD.CD45.2 (#014149; Jax), and NOD.Foxp3/GFP mice (#025097; Jax) were obtained from The Jackson Laboratory. NOD.Foxp3/GFP mice were bred with BDC2.5 mice and NOD.CD45.2 mice to produce BDC2.5.Foxp3/GFP mice with CD45.2 and/or CD45.1 congenic markers for in vivo tracking. All mice were bred and maintained in the specific pathogen-free animal barrier facility of the Columbia Center for Translational Immunology. Both male and female transgenic/congenic mice (6–16 weeks of age) were used as donors for adoptive transfer experiments. Male and female NOD mice (8–12 weeks of age) were used as recipient mice for adoptive transfer and in vivo uptake studies. Female NOD mice (8 weeks of age) were used in studies involving treatments (preclinical) and MHC tetramers (Tetrs). All procedures were approved by the Columbia University Institutional Animal Care and Use Committee and performed in adherence with the National Institutes of Health Guide for the Care and Use of Laboratory Animals.
Nod foxp3 gfp mice
Manufactured by Jackson ImmunoResearch
The NOD.Foxp3/GFP mice are a genetically modified mouse strain that expresses green fluorescent protein (GFP) under the control of the Foxp3 gene promoter. Foxp3 is a key transcription factor that is essential for the development and function of regulatory T cells (Tregs). The expression of GFP in these mice allows for the identification and isolation of Tregs for research purposes.
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Lab products found in correlation
2 protocols using nod foxp3 gfp mice
1
Adoptive Transfer of Transgenic/Congenic NOD Mice
2
Generation and Characterization of Genetically Modified Mouse Models
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B6 and NOD/Foxp3GFP mice (Haribhai et al., 2007 (link)) were purchased from Jackson laboratory. NOD and NOD/BDC2.5/Thy1.1 mouse lines were obtained from Mathis-Benoist laboratory (Hill et al., 2007 (link)). NOD/BDC2.5/Thy1.1 mice were further crossed to NOD/Foxp3GFP to generate NOD/BDC2.5/Foxp3GFP/Thy1.1+ and NOD/BDC2.5/Foxp3GFP Thy1.1+ Thy1.2+ mice. B6/CRIg KO mice were provided by Genentech (Helmy et al., 2006 (link)). NOD/CRIg KO mice were generated by crossing B6/CRIg KO mice onto a NOD background for more than 10 generations. Speed-congenics was used to facilitate the generation of NOD/CRIg KO mice. All mice were housed under specific pathogen free (SPF) conditions in our animal facility at University of California, San Diego, in accordance with the ethical guidelines of the Institutional Animal Care and Use Committee.
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