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Gatan 914 high tilt cryo holder

Manufactured by Ametek
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Sourced in Japan, United States

The Gatan 914 high tilt cryo holder is a specimen holder designed for use in electron microscopy. It is capable of maintaining a cryogenic temperature to allow for the observation of specimens in a frozen state.

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Cryo-holder (26 citations) 914 cryo-holder (6 citations) Gatan 914 (3 citations)

2 protocols using gatan 914 high tilt cryo holder

1

Cryo-TEM Imaging of Extracellular Vesicles

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EV suspensions obtained by ultracentrifugation were resuspended in 20 µl EV-free PBS. Gold R2/2 Quantifoil grids were cleaned for 40 seconds using a Cressington 208 coater. The sample was applied on a grid and subsequently vitrified in liquid ethane using a FEI Vitrobot device. A JEOL JEM-2100 system (Jeol Ltd., Tokyo, Japan) equipped with Gatan 914 high tilt cryo holder and a LaB6 filament was used for cryogenic imaging at 200 kV. Images were recorded with a Gatan 833 Orius camera (Pleasanton, CA, USA).
Carotti V., van der Wijst J., Verschuren E.H., Rutten L., Sommerdijk N., Kaffa C., Sommers V., Rigalli J.P, & Hoenderop J.G. (2022). Involvement of ceramide biosynthesis in increased extracellular vesicle release in Pkd1 knock out cells. Frontiers in Endocrinology, 13, 1005639.
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2

Cryo-EM Imaging of Biomolecular Complexes

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Frozen electron microscopy grids were mounted on a Gatan 914 high-tilt cryo-holder (Gatan, Pleasanton, CA, USA). Cryo-EM images were collected on JEOL 2200FS 200kV field emission gun electron microscope (JEOL, Tokyo, Japan). 2k by 2k images were collected using a Gatan US1000 slow-scan CCD camera. Energy-filtered (20 eV slit) images were collected at pixel sizes between 0.54 and 0.72 angstrom at the specimen level with nominal defocus between 5 and 3 μm depending on the experiment.
Gomord V., Stordeur V., Fitchette A.C., Fixman E.D., Tropper G., Garnier L., Desgagnes R., Viel S., Couillard J., Beauverger G., Trepout S., Ward B.J., van Ree R., Faye L, & Vézina L.P. (2020). Design, production and immunomodulatory potency of a novel allergen bioparticle. PLoS ONE, 15(12), e0242867.
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