P 2000 polarimeter

Manufactured by Jasco

Sourced in Japan, United States, Italy, Germany

The P-2000 polarimeter is a laboratory instrument used to measure the optical rotation of a sample. It determines the angle of rotation of the plane of polarized light passing through a sample, which can be used to analyze the chemical composition or concentration of chiral molecules.

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Lab products found in correlation

Sephadex lh 20, by GE Healthcare (17 mentions) Silica gel 60, by Merck Group (13 mentions) Ft ir 4100 spectrometer, by Jasco (13 mentions) V 650 spectrophotometer, by Jasco (11 mentions) 6220 lc tof ms, by Agilent Technologies (9 mentions) Avance 500 spectrometer, by Bruker (8 mentions) 6130 quadrupole mass spectrometer, by Agilent Technologies (8 mentions) Du800 spectrophotometer, by Beckman Coulter (7 mentions) Rp 18 f254, by Merck Group (7 mentions) Ft ir 4200 spectrometer, by Jasco (6 mentions) Uv 1201 spectrophotometer, by Shimadzu (6 mentions) Sephadex lh 20, by Merck Group (6 mentions) Silica gel 60 f254, by Merck Group (6 mentions) Nicolet 6700 ft ir spectrometer, by Thermo Fisher Scientific (6 mentions) Avance 3 spectrometer, by Bruker (6 mentions) Tci microcryoprobe, by Bruker (6 mentions) Agilent 1100 dad, by Agilent Technologies (6 mentions) Rp 18 f254s plates, by Merck Group (5 mentions) Lc 8a pump, by Shimadzu (5 mentions) Cosmosil 5c18 ar 2 column, by Nacalai Tesque (5 mentions)

Spelling variants of this product

P-2000 digital polarimeter (45 citations) P-2000 (40 citations) P-2000 series polarimeter (2 citations)

220 protocols using p 2000 polarimeter

Enzymatic Hydrolysis and Optical Rotation Determination

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The absolute configurations were determined for the residual EO and the product OD obtained by this hydrolytic enzyme reaction. Briefly, 100 mM 1,2-EO (100 mg) and 100 µg recombinant Yleh enzyme were incubated in 10 mL 50 mM Tris-sulphate buffer, pH 8.0, and the reaction mixture was incubated at 30 °C for 1 h with gentle shaking. The reaction was stopped, and the remaining 1,2-EO was extracted with an equal volume of chilled pentane (Weijers et al. 1998 (link)). The organic layer was dried with MgSO₄ salt and concentrated by evaporation to measure the specific optical rotation values.
For the absolute configuration of the reaction product (1,2-OD), from the same hydrolytic reactions, the remaining aqueous reaction mixture was extracted twice with an equal volume of ethyl acetate (1:1 v/v). The organic layer was dried with MgSO₄ salt and evaporated under reduced pressure to give an oily residue of product OD. The concentrated diol was re-dissolved in methanol and used to measure specific optical rotation values. Optical rotation values were measured on a P-2000 Jasco Polarimeter at 589 nm, and data were analyzed using the Spectra Manager™ software provided with the instrument.

Godase V.P., Kumar V.R, & Kumar A.R. (2023). Potential of Y. lipolytica epoxide hydrolase for efficient production of enantiopure (R)-1,2-octanediol. AMB Express, 13, 77.

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Nuclear Magnetic Resonance Spectroscopy Protocol

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Proton nuclear magnetic resonance (¹H NMR) spectra were recorded with a Bruker Avance III 500 MHz spectrometer, are reported in parts per million, and are referenced to the residual protium in the NMR solvent (CDCl₃: δ 7.24 (CHCl₃), CD₃OD: δ 3.31 (CHD₂OD), DMSO-d₆: δ 2.50 (DMSO-d₅)). Data are reported as follows: chemical shift [multiplicity (s = singlet, d = doublet, t = triplet, sp = septet, m = multiplet), coupling constant(s) in Hertz, integration]. Carbon-13 nuclear magnetic resonance (¹³C NMR) spectra were recorded with a Bruker Avance III 500 MHz spectrometer, are reported in parts per million, and are referenced from the carbon resonances of the solvent (CDCl₃: δ 77.23, CD₃OD: δ 49.15, DMSO-d₆: δ 39.51). Data are reported as follows: chemical shift. Infrared data (IR) were obtained with a Cary 630 Fourier transform infrared spectrometer equipped with a diamond ATR objective and are reported as follows: frequency of absorption (cm⁻¹), intensity of absorption (s = strong, m = medium, w = weak, br = broad). Optical rotations were measured on a P-2000 JASCO polarimeter and compound concentrations are expressed in units of g/100 mL. High resolution mass spectra (HRMS) were recorded by the Harvard University Small Molecule Mass Spectrometry facility on an Agilent 6210 time-of-flight LCMS using an electrospray ionization (ESI) source.

Rivera S.L., Espaillat A., Aditham A.K., Shieh P., Muriel-Mundo C., Kim J., Cava F, & Siegrist M.S. (2020). Chemically-Induced Cell Wall Stapling in Bacteria. Cell chemical biology, 28(2), 213-220.e4.

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Spectroscopic Characterization of Organic Compounds

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IR spectrum was obtained using a Thermo Electron (Nicolet 380) FT-IR spectrometer. Column chromatography was carried out using silica gel (Merck 60-120, 70-230 and 230-400 mesh). Rotating power was recorded on a P-2000 Jasco polarimeter. Thin layer chromatography was performed on percolated 0.5mm thickMerck Si gel 60 F254 aluminium sheets. Separated compounds were visualized under UV light and by spraying with H2SO4-EtOH (1:9, v/v) followed by mild heating for about 2-3 min. The mass spectra were recorded on an Agilent MS instrument (Agilent Technologies 6520, Accurate mass Q-Tof). NMR spectra were recorded on a Bruker Avance DRX-500 instrument operating at 500 MHz ( 1 H) and 125 MHz ( 13 C), using a deuterated dimethylsulfoxyde (DMSO-d6) as solvent. Chemical shifts () were quoted in parts per million (ppm) from internal standard tetramethylsilane (TMS) and the coupling constants (J) are given in Hz. Different mixtures of n-hexane, EtOAc, CH2Cl2 and MeOH were used as eluting solvents. They were distilled prior to use.

Toussi Matchi J.L., Tchamo Noungoue D., Kuhn I., Boissier J., Tchouankeu J.C., Nothisen M., Chaubet G., Garnier D., Ursuegui S., Ngouela S.A, & Wagner A. (2021). Manniindole, an indole derivative from the roots of Anonidium mannii and combined antischistosomal and enzymatic activities. Natural product research, 35(24).

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Phytochemical Analysis of Natural Products

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Optical rotations were measured on P2000 polarimeter (JASCO, Tokyo, Japan). UV spectra were obtained on a JASCOP650 spectrometer (JASCO). IR spectra were recorded on a Nicolet 5700 FT-IR microscope instrument (FT-IR microscope transmission, Thermo Electron Corporation, Madison, WI, USA). 1D and 2D NMR spectra were acquired at 500 or 600 MHz for ¹H and 125 or 150 MHz for ¹³C, respectively, on Varian INOVA 500 MHz, or Bruker AVANCE III HD 600 MHz (Bruker Corporation, Karlsruhe, Germany), in acetone-d₆ or methanol-d₄, with solvent peaks as references. ESI-MS and HR-ESI-MS data were measured using an AccuToFCS JMST100CS spectrometer (Agilent Technologies, Ltd., Santa Clara, CA, USA). Column chromatography (CC) was performed with silica gel (200–300 mesh, Qingdao Marine Chemical Inc., Qingdao, China). HPLC separation was performed on an instrument consisting of a Waters 515 pump and a Waters 2487 dual λ absorbance detector (Waters Corporation, Milford, MA, USA) with a YMC semi-preparative column (250 × 10 mm i.d.) packed with C18 (5 μM). TLC was carried out with glass precoated silica gel GF254 plates (Qingdao Marine Chemical, Inc., Qingdao, China). Spots were visualized under UV light or by spraying with 7% H₂SO₄ in 95% EtOH followed by heating.

Zhang J.Q., Li G.P., Kang Y.L., Teng B.H, & Yao C.S. (2017). Biomimetic Synthesis of Resveratrol Trimers Catalyzed by Horseradish Peroxidase. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(5), 819.

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Isolation and Characterization of Compounds

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Preparative high performance liquid chromatography (HPLC) was performed using the Phenomenex Luna C18 column (100A, 250 × 21.2 mm, 5 sm) by using the Waters 2695 Separations Module, and the eluate was monitored at a UV wavelength of 210 nm (Waters 2998 Photodiode Array Detector; Milford, United States). ¹H NMR was performed on the 500 and the 800 MHz Varian spectrometers, and ¹³C NMR spectra were obtained on the 200 MHz Varian spectrometers. Standard 2D NMR experimental spectra, including HSQC, heteronuclear multiple bond correlation (HMBC), and COSY, were collected at 25°C. MS data were recorded from the Bruker ultrafleXtreme ultrahigh-resolution TOF LC-MS system. Optical rotations were determined using the Jasco P-2000 Polarimeter. Circular dichroism spectra were obtained using the Chirascan circular dichroism spectrometer.

She W., Ye W., Cheng A., Liu X., Tang J., Lan Y., Chen F, & Qian P.Y. (2021). Discovery, Bioactivity Evaluation, Biosynthetic Gene Cluster Identification, and Heterologous Expression of Novel Albofungin Derivatives. Frontiers in Microbiology, 12, 635268.

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Analytical Characterization of Compounds

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Optical rotation was measured using a JASCO P-2000 polarimeter (Tokyo, Japan). The NMR spectra were acquired on a 400 MHz Agilent NMR spectrometer (DD2, Santa Clara, CA, USA) using CDCl₃. The HRESIMS was performed on an Agilent 6220 Accurate-Mass TOF LC/MS system. Silica gel (230–400 mesh, Merck KGaA, 64271 Darmstadt, Germany) and RP-18 (YMC gel ODS-A, 12 nm, S-150 μm, YMC Co., Ltd., Kyoto 600-8106, Japan). Thin Layer Chromatography (TLC) analysis was performed on silica gel 60 F254 (0.2 mm thickness, Merck KGaA, 64271 Darmstadt, Germany) and RP-18 F 254_S (Merck KGaA, 64271 Darmstadt, Germany) plates by visualization under UV light at 254 nm, 365 nm, and 10% (v/v) of sulfuric acid followed by heating. Preparative HPLC was performed using an Acme 9000 system (Young Lin, Anyang, Korea) equipped with a YMC-Pack Pro C18 column (5 μm, 250 × 20 mm i.d.).

Thapa P., Lee Y.J., Nguyen T.T., Piao D., Lee H., Han S., Lee Y.J., Han A.R., Choi H., Jeong J.H., Nam J.W, & Seo E.K. (2021). Eudesmane and Eremophilane Sesquiterpenes from the Fruits of Alpinia oxyphylla with Protective Effects against Oxidative Stress in Adipose-Derived Mesenchymal Stem Cells. Molecules, 26(6), 1762.

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Analytical Techniques for Natural Product Isolation

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Silica gel and silica gel C₁₈, both 40-63 μm, were
used for TLC and the C₁₈ solid phase extraction. HPLC isolations were
conducted with a Shimadzu SCL-10A VP controller, SPD-M10A VP photodiode array
detector and two LC-10AT VP pumps with a 250 × 4.60 mm, Luna 5 micron,
C₁₈ Phenomenex column. UV and IR spectra were measured on
Shimadzu UV-1201 and Bruker Tensor 27 FTIR spectrometers. Optical rotation was
recorded on a JASCO P-2000 polarimeter. The mass spectrum was obtained on an
Agilent 6220 LC/MS TOF. ¹H- and ¹³C-NMR data were recorded
on a Bruker 600 spectrometer in CDCl₃ with TMS as an internal
standard.

Presley C.C., Rakotondraibe L.H., Brodie P.J., Callmander M.W., Randrianaivo R., Rasamison V.E., Rakotobe E, & Kingston D.G. (2015). A Synthetic Butenolide Diterpene is now a Natural Product Isolated from Metaporana sericosepala, a Plant from the Madagascar Dry Forest[]. Natural product communications, 10(9), 1505-1507.

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Spectroscopic Characterization of Organic Compounds

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Optical rotation was measured on a Jasco P-2000 polarimeter (JASCO Analytical Instruments, Easton, MD, USA). ¹H and ¹³C NMR spectra as well as 2-D NMR data were collected on a Varian vs800 instrument (¹H NMR referenced to the HOD peak at δ_H 4.71; ¹³C NMR was referenced to residual MeOH in D₂O at δ_C 49.5).⁴⁶ LC-MS data was obtained on a Thermo-Electron LTQ LC/MS instrument with a Phenomenex Luna 5 μm C18(2) 100 Å column (4.6 × 250 mm) and a gradient starting at 60% MeCN/40% H₂O and immediately ramping to 100% MeCN over 20 min, then holding at 100% MeCN for 5 min. MS fragmentation experiments were obtained using a Biversa Nanomate (Advion Biosystems) electrospray source for a Finnigan LTQ-FTICR-MS instrument (Thermo-Electron Corporation) running Tune Plus software version 1.0. HPLC purification was carried out with a Waters 515 HPLC pump with a Waters 996 photodiode array detector using Empower Pro software and a Phenomenex Synergi 4 μm C18 column (10 × 250 mm) and a gradient starting at 50:50 MeCN/H₂O for 10 min, ramping to 100% MeCN over 12 min and holding for 3 min. All solvents were HPLC grade.

Kinnel R.B., Esquenazi E., Leao T., Moss N., Mevers E., Pereira A.R., Monroe E.A., Korobeynikov A., Murray T.F., Sherman D., Gerwick L., Dorrestein P.C, & Gerwick W.H. (2017). A Maldiisotopic Approach to Discover Natural Products: Cryptomaldamide, a Hybrid Tripeptide from the Marine Cyanobacterium Moorea producens. Journal of natural products, 80(5), 1514-1521.

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NMR Spectroscopy and Mass Spectrometry Analysis

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¹H and ¹³C nuclear magnetic resonance spectra were recorded with Bruker AVANCE NEO (500 MHz), Bruker AVANCE III HD Nanobay (400 MHz), Bruker AVANCE III HD (400 MHz), or Bruker AVANE NEO Nanobay (400 MHz) and calibrated by using the residual undeuterated chloroform (δ_H = 7.24 ppm) and CDCl₃ (δ_C = 77.23 ppm) or monodeuterated dichloromethane (δ_H = 5.32 ppm) as internal references. Data are reported in the following manners: chemical shift in ppm [multiplicity (s = singlet, d = doublet, t = triplet, q = quartet, p = quintet, m = multiplet, app = apparent, br = broad), coupling constant(s) in Hertz, integration]. The NMR solvent CDCl₃ was taken from a stock containing anhydrous K₂CO₃ to remove residual DCl. High resolution mass spectra were obtained from KAIST Analysis Center for Research Advancement (Daejeon) by using ESI ionization method. Specific rotation was obtained by JASCO P-2000 polarimeter.

Park S., Kang G., Kim C., Kim D, & Han S. (2022). Collective total synthesis of C4-oxygenated securinine-type alkaloids via stereocontrolled diversifications on the piperidine core. Nature Communications, 13, 5149.

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Spectroscopic Analysis and Chromatographic Techniques

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NMR spectra were recorded on a Bruker AVANCE II+ 600 NMR spectrometer operating at 600 MHz (150 MHz for ¹³C). Optical rotation was measured on a Jasco P-2000 polarimeter. Vacuum liquid chromatography (VLC) was performed on Silica gel 60H (Merck, 15 μm). Column chromatography (CC) was performed on Silica gel 60 (Merck, 63–200 μm) normal phase, and Sephadex LH-20 (Pharmacia Fine Chemicals, 25–100 μm). Low pressure liquid chromatography (LPLC) was carried out with LiChroprep Si 60 Merck column (40–63 μm). Preparative thin-layer chromatography (prep. TLC) was performed on Silica gel 60 F₂₅₄ glass plates (Merck, 20 x 20 cm; 0.25 mm). Detection of the spots was achieved under UV light at 254 and 366 nm, and by spraying with vanillin in sulfuric acid, followed by heating at 100°C. All solvents used were of analytical grade.

Georgieva K., Popova M., Dimitrova L., Trusheva B., Thanh L.N., Phuong D.T., Lien N.T., Najdenski H, & Bankova V. (2019). Phytochemical analysis of Vietnamese propolis produced by the stingless bee Lisotrigona cacciae. PLoS ONE, 14(4), e0216074.

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