The thoracic aortal and left ventricular samples from WKY, SHR, and SHR-PASE groups were fixed in 10 % formalin and then paraffin-embedded for H&E or Masson staining. The sections were examined under light microscope at ×200 magnification using the Olympus image analysis software system (Olympus America, Center Valley, PA, United States). The percentage of collagen in the aortal space, myocardial interstitial space, or around the small artery of left ventricle was calculated using Image-pro Plus 6.0 software (Media Cybernetics, Inc., Rockville, MD, United States).
Image analysis software system
Manufactured by Olympus
Sourced in United States
The Olympus image analysis software system is a comprehensive suite of tools designed for efficient digital image processing and analysis. The software provides a range of functionalities for capturing, managing, and analyzing images from various sources, including microscopes and other imaging devices. The core function of the system is to enable users to extract valuable information and insights from digital images through advanced image processing algorithms and analytical tools.
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Lab products found in correlation
3 protocols using image analysis software system
1
Quantifying Collagen in Aorta and Ventricle
2
Histological Analysis of Liver, WAT, and BAT
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Fresh liver, WAT, and BAT samples were fixed in 10% formaldehyde and then paraffin-embedded for H&E staining. Another portion of liver tissue was fixed in 4% paraformaldehyde and embedded in OCT (Sakura Finetek, Torrance, CA, USA) for oil red O (Sigma-Aldrich, St. Louis, MO, USA) staining. The sections were examined under light microscope (×200) by using the Olympus image analysis software system (Olympus America, Center Valley, PA, USA).
3
Histological Analysis of Frozen WAT
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Frozen white adipose tissue (WAT) samples were fixed in 10% formaldehyde and paraffin-embedded. The sections were stained with hematoxylin and eosin and examined under the light microscope (×200) using the Olympus image analysis software system (Olympus America, Melville, NY, USA). Their surface areas were acquired with Olympus CellSens Standard software (Version 1.5).
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