Rabbit anti β actin ac026

Rabbit anti-RSPH9 (23253-1-AP) and anti-CENTRIN1 (12794-1-AP) were obtained from
Proteintech (Wuhan, China). Rabbit anti-β-Actin (AC026) and mouse anti-β-Tubulin (AC021)
were from Abclonal (Wuhan, China). Rabbit anti-Acetylated Tubulin was purchased from Cell
Signaling Technology (MA, USA). Mouse anti-FLAG M2 (F3165), anti-Acetylated Tubulin
(T6793) and anti-α-Tubulin (T9026) were from Sigma-Aldrich (St. Louis, MO,USA). Mouse
anti-HA-tag (M180–3) and mouse anti-DDDDK-tag (PM020) were from Medical & Biological
Laboratories (Nagoya, JP).
Antibodies specific for DRC1, CCDC65, LRRC48 and GAS8 were prepared as per previously
published protocols (59 (link)). Briefly, murine DRC1
(aa 1–146), CCDC65 (aa 1–126), LRRC48 (aa 182–300) and GAS8 (aa 1–478) were expressed as
His fusion proteins in Escherichia coli using the pET-28a (+) vector,
after which the Ni-NTA His Bind Resin was used to affinity purify these proteins. For mice
were then immunized with the resultant fusion proteins, yielding the four following
antiserum preparations: anti-DRC1, anti-CCDC65, anti-LRRC48 and anti-GAS8.

Mouse anti-Flag (F1804) and rabbit anti-HA (H6908) antibodies were purchased from Sigma-Aldrich (St Louis, MO, USA). Rabbit anti-GFP (50430-2-AP), Rabbit anti-LC3 (14600-1-AP) and Rabbit anti-P62 Polyclonal antibody(18420-1-AP) were purchased from Proteintech Group (Chicago, IL, USA). Rabbit anti-β-actin (AC026) and anti-EV71-3D polyclonal antibodies were produced by ABclonal Technology (Wuhan, China). Rabbit anti-EV71-VP1 (PAB7631-D01P) polyclonal antibody was purchased from Abnova (Taipei city, Taiwan). Rabbit anti-Beclin1 (381896) antibody was purchased from ZENBIO (Chengdu, China). Delbecco modified Eagle medium (DMEM), and fetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, USA). Lipofectamine 2000, normal rabbit IgG and normal mouse IgG were purchased from Invitrogen Corporation (Carlsbad, CA, USA). Protein ladder (26616) was purchased from Thermo scientific (Rockford, IL, USA). Complete, EDTA-free Protease Inhibitor Cocktail was purchased from Roche (Basel, Switzerland). Chloroquine diphosphate were purchased from Selleck (Houston, TX, USA). Rapamycin (53123-88-9) was obtained from Target Mol (Shanghai, China).