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7 protocols using sucrose

1

Sucrose Intake Test Protocol

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To evaluate the effects of treatments in sucrose intake test, the reported procedure was followed.[23 (link)] sucrose (1%; LobaChemie Private Limited, Mumbai, India) was dissolved in drinking water and animals were trained to drink sucrose solutions during 2 days with free access to water and sucrose in their home cage, and the total fluid consumption per day was measured. Then they were deprived of any drinking solution for 24 h and subsequently each animal was given free access to sucrose solutions for 1 h in its home cage. The milliliters (ml) of sucrose consumed by each rat were recorded and the mean sucrose intake was compared.
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2

Formulation and Evaluation of LCD

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Levocetirizine dihydrochloride (LCD) was obtained as a gift sample from Modern Pharmaceutical Company, Sana'a, Yemen; HPMC and propylene glycol were purchased from Sigma-Aldrich, UK; sodium starch glycolate (SSG), croscarmellose sodium (CCS), crospovidone (CP), and gelatine were purchased from SD Fine Chemicals, India; sucrose, vanilla, and citric acid were purchased from Loba Chemie, India. All chemicals used were of analytical grade.
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3

Quantitative Analysis of Sugars and 5-HMF

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Chemicals and reagents: All the chemicals and reagents employed were of high-performance liquid chromatography (HPLC) ranking. Fructose, glucose, sucrose, and maltose were acquired from Loba Chemie Pvt. Ltd. Mumbai, India; Chem-Lab NV, Zedelgem, Belgium; Central Drug House (P) Ltd, New Delhi, India; and Techno Pharmchem, New Delhi, India respectively. 5-(hydroxymethyl) furfural were obtained from Sigma-Aldrich (St. Louis, MO, USA). Water and Acetonitrile, HPLC grade were obtained from Chemie Pvt. Ltd. Mumbai, India.
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4

Multiparametric Evaluation of Metabolic Disorders

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Sodium citrate (Guangzou jhd Chemical Reagents Co., Ltd, China), citric acid, (Guangzou jhd Chemical Reagents Co., Ltd, China), streptozotocin (Sigma-Aldrich, Germany), nicotinamide (Qualigens Fine Chemicals, GSK India), D-glucose (Loba Chemie Pvt Ltd, Mumbai), sucrose (Loba Chemie Pvt Ltd, Mumbai), metformin (Bristol-Myers Squibb, New York, United State), glibenclamide (Sanofi Aventis, Paris, France), orlistat (GlaxoSmithKline), glucometer and strip (Roche Diagnostics, Rotkreuz, Switzerland). Triglyceride, cholesterol, high-density lipoprotein, glutathione, catalase, superoxide dismutase and 2-ThioBarbituric Acid Reactive Substances (TBARS) kits were products of Fortress Diagnostic, United Kingdom. Insulin and leptin kits were products of Crystal Chrom, Drowner, IL, United State, adiponectin kit (enzyme immunoassay (EIA)) is a product of Ray Biotech, Norcross, GA, USA, and serum lipase colorimetric kit is a product of BioAssay Systems, Hayward, CA, USA.
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5

Biofilm Formation in Tryptone Soya Broth

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Tryptone soya broth (HiMedia Laboratories, India) was used for biofilm formation. Media were prepared according to the manufacturer’s instructions before autoclaving at 121 °C for 15 min. All chemicals used throughout the current study were of analytical grade. Sucrose was a product of Loba Chemie, India. Fluor defender (0.1% fluorosilane equivalent to 1600 ppm fluoride) is a fluoride varnish produced by Cerkamed Co., Poland. Enamelast® (5% sodium fluoride equivalent to 22,600 ppm fluoride) is a product of Ultradent Co., USA.
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6

Electrochemical Sensing of Biomolecules

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All chemicals used were of analytical grade. Ascorbic acid (AA, 99%), disodium hydrogen phosphate (Na2HPO4, 99.5%), dopamine (DA, 99%), D-( + )-glucose (Glu, 99.5%), uric acid (UA, 99%), L-potassium chloride (KCl, 99.5%), sodium chloride (NaCl, 99.5%), sodium dihydrogen phosphate (NaH2PO4, 99.5%), and human serum from human male (AB plasma, USA origin) were purchased from Sigma-Aldrich, Singapore. Carbon ink was purchased from Acheson, Singapore. Natural graphite ( 98%) was purchased from Bay Carbon Inc., USA. Sucrose (Su, extra pure), sodium hydroxide (NaOH, 98%), and copper (II) nitrate (Cu(NO3)2, 99.5%) were purchased from Loba Chemie, India. Potassium ferricyanide (K3[Fe(CN)6], 99%), and diethylene glycol monobutyl ether (HOCH2CH2OCH2CH2O (CH2)3CH3, 98.0%) were obtained from Merck, Germany. Deionized water (DI water, 18.2 MΩ cm at 25 C ) was collected from a purification system (Millipore systems, USA).
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7

Germination of Green Immature Seeds

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Healthy green pods were surface disinfected with chorine based commercial bleach solution (2.5% (v/v, Clorox Co. Saudi Arabia) containing 0.05% (v/v) Tween 20. With sterile purified water, the pods were washed 4–5 times after 10 min to eliminate all the traces of bleach. Green immature seeds were aseptically isolated from the sterilized pods and planted on different strengths of growth hormones free Nitsch medium (Nitsch and Nitsch, 1969 (link); Duchefa BV, Haarlem, The Netherlands) (pH 5.7) supplied with 3% (w/v) sucrose (LOBA Chemie, Mumbai India) and 0.7% (w/v) agar (SIGMA-ALDRICH, Spain) before being sterilized at 15 psi (121 °C) for 20 min in a steam-based ALP-autoclave (CLG-32L, ALP Co., Tokyo, Japan). All the cultured plates were incubated at 24 ± °C in dark for 48 h and after that they were transferred under light (16/8h light–dark cycle) with luminous intensity of 50 µmol m-2s−1 in a growth chamber (Conviron Adaptis, USA) provided by the cool LED lamps.
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