For MS1-induction experiments, bone marrow or peripheral mononuclear cells were cultured in SFEM II supplemented with 1X CC110 (StemCell Technologies) with or without the presence of 100 ng/mL LPS or Pam3CSK4 (Invivogen) for up to 4 days. For re-stimulation experiments, sorted monocytes were rested for 24 hours in RPMI-1640 supplemented with 10% heat-inactivated FBS and 1X Pen-Strep (Gibco), before adding 100 ng/mL LPS (Invivogen).
Cc110
Manufactured by STEMCELL
The CC110 is a laboratory instrument designed for automated cell counting and viability analysis. It utilizes advanced optical imaging technologies to accurately measure the number and viability of cells in a sample. The core function of the CC110 is to provide reliable and consistent cell count and viability data to support various cell-based research and applications.
Automatically generated - may contain errors
Lab products found in correlation
Sfemii, by STEMCELL (4 mentions)
Pen strep, by Thermo Fisher Scientific (2 mentions)
Pam3csk4, by InvivoGen (1 mentions)
Lipopolysaccharides (lps), by InvivoGen (1 mentions)
Stemspan sfem 2 medium, by STEMCELL (1 mentions)
Stemregenin 1, by STEMCELL (1 mentions)
Um171, by STEMCELL (1 mentions)
Cd34 hspc, by Lonza (1 mentions)
Recombinant human il 6, by Thermo Fisher Scientific (1 mentions)
Synthemax, by Corning (1 mentions)
Human il 2, by Miltenyi Biotec (1 mentions)
X vivo 15 media, by Lonza (1 mentions)
Cryostor cs5, by STEMCELL (1 mentions)
Cd3 cd28 dynabead, by Thermo Fisher Scientific (1 mentions)
Human ab serum, by Gemini Bio (1 mentions)
Easysep human t cell enrichment kit, by STEMCELL (1 mentions)
Rapamycin, by Merck Group (1 mentions)
Chir99021, by Merck Group (1 mentions)
5 protocols using cc110
1
Monocyte Differentiation and Activation
2
Hematopoietic Stem Cell Genome Editing
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human bone marrow CD34+ hematopoietic stem/progenitor cells were obtained from AllCells and Fred Hutchinson Cancer Research Center. CD34+ cells were maintained in StemSpan SFEM II medium supplemented with CC110 (StemCell Technologies). K562 cells were cultured in IMDM supplemented with 10% FBS and 1% penicillin/streptomycin. Frozen stocks were prepared with 10% DMSO and 30% FBS in K562 medium and stored in liquid nitrogen prior to use. Genome editing experiments in hESCs were performed as previously described (Hockemeyer et al,2009 ; Soldner et al,2009 ; Hockemeyer et al,2011 ) in the WIBR#3 line (Lengner et al,2010 ) and NIH stem cell registry # 0079. Cell culture was carried out as previously described in Boyle et al (2020 ).
3
Expansion of CD34+ HSPCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All human tissues used in this study received informed donor consent and their usage was tracked internally by Novartis Institutes for BioMedical Research (NIBR) Human Tissue Network. All experimental protocols involving human tissues were approved by NIBR Human Tissue Council, and all experiments involving human tissues were executed in full compliance to NIBR Human Tissue Council Research and Quality guidelines. Cryopreserved bone marrow derived CD34+ HSPCs were purchased from Lonza or AllCells and thawed according to vendors’ instructions. Cells were cultured at 37 °C 5% CO2 in SFEM II supplemented with CC110 (StemCell Technologies), 0.75 μM StemRegenin 1 (StemCell Technologies), 50 nM UM171 (StemCell Technologies), 50 ng/mL human recombinant IL-6 (Peprotech), and Pen-Strep (Life Tech 15140-122). Cells were cultured for 3–5 days before being used in experiments.
4
Comprehensive Protocol for T-cell Culture and Editing
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CD34 + HSPCs were cultured in SC media (SFEMII and CC110 (STEMCELL Techonologies)). CD4 + T cells were purified from frozen human peripheral blood Leukopak (STEMCELL Techonologies) by negative selection using an EasySep Human T Cell Enrichment Kit (STEMCELL Technologies) according to the manufacturer’s instructions and cryopreserved in CryoStor CS5 (STEMCELL Technologies). Purified T cells were cultured in X-VIVO 15 Media (Lonza) supplemented with 5% human AB serum (GeminiBio) and 100†‰IU ml âˆ'1 human IL-2 (Miltenyi Biotec). For gene editing experiments, T cells were activated using TransAct (Miltenyi Biotec) according to the manufacturer’s instructions. For Fig. 4e,f, T cells were activated 1 d after thaw using CD3/CD28 Dynabeads (Thermo Fisher Scientific) according to the manufacturer’s instructions. The beads were removed after 2 d. The T cells were used in gene editing experiments on either day 3 or day 4. iPSCs were cultured in complete StemFlex media (Gibco Life Technologies) and seeded in the coated plates with Synthemax (Corning).
5
Hematopoietic Stem Cell Culture Media
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!