A Hitachi TM3000 (Hitachi High-Technologies, Tokyo, Japan) SM was used to visualize the surface structure of dried potato samples. A small layer of dried potato powder was mounted on a thin layer of carbon tape. Images were captured using TM3000 software (Hitachi High-Technologies, Version 02-03, Tokyo, Japan) using “Compo” and “Shadow1” image modes at 100×, 500× and 1000× magnification and obtained at 5 kV.
Tm3000
Manufactured by Hitachi
Sourced in Japan, United States
The TM3000 is a tabletop scanning electron microscope (SEM) produced by Hitachi. The core function of the TM3000 is to provide high-resolution imaging of samples at the microscopic level.
Automatically generated - may contain errors
Lab products found in correlation
Quantax 70, by Bruker (8 mentions)
S 4800, by Hitachi (7 mentions)
Jem 2100, by JEOL (5 mentions)
Swifted3000, by Hitachi (3 mentions)
Ultima 4, by Rigaku (3 mentions)
Physical property measurement system (ppms), by Quantum Design (3 mentions)
Nicolet is10, by Thermo Fisher Scientific (3 mentions)
E 1010, by Hitachi (2 mentions)
Em cpd300, by Leica (2 mentions)
Su 70, by Hitachi (2 mentions)
Smz1500, by Nikon (2 mentions)
Sc7620, by Quorum Technologies (2 mentions)
Tg 209 f1, by Netzsch (2 mentions)
Su5000, by Hitachi (2 mentions)
Su8020, by Hitachi (2 mentions)
Tabletop microscope, by Hitachi (2 mentions)
Eos 700d, by Canon (2 mentions)
Ft ir 470 plus, by Jasco (2 mentions)
E 1045, by Hitachi (2 mentions)
Miniflex 2, by Rigaku (2 mentions)
333 protocols using tm3000
1
Potato Surface Structure Visualization
2
Biodistribution of Aptamer-Functionalized Nanoparticles
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After injection of AS-14-GMNPs in the tail vein, its distribution in the tumor and other organs was analyzed using electron microscopy on 30μm tissue sections. On the7th day after the tumor transplantation, the mice were injected with GMNPs functionalized with FAM-labeled aptamer AS-14 in 100 μL DPBS (1.6 μg kg-1). After 1, 5 and 24 hours the animals were euthanized and the tumor, liver, kidney and urine were harvested, 30μm tissue sections were prepared using cryostat HM 525 (Carl Zeiss, Germany), fixed on glass slides and placed on silicon foil. Electron microscopy (Hitachi TM3000, Japan) was used to visualize and estimate the percentage ratios of iron and gold. Electron microscopy spectra were processed with the Quantax 70 software (Bruker) for Hitachi TM3000.
3
Visualizing Protein-Aptamer Interactions
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An electron microscopy (Hitachi TM3000, Japan) was used to visualize the sandwich formation of cancer-related proteins bound with LC-18 aptamer on the electrodes surfaces with IH-SAB 4C–8C beads. In order to estimate percentage ratios of molecules present on the electrodes EM spectra were processed with the software Quantax 70 (Bruker) for Hitachi TM3000.
4
Morphological Analysis of Pollen Grains
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The freshly unopened flower samples of male sterile and male fertile plants were gathered from the field in the early dawn,and put away in impenetrable zip lock polythene covers over the ice package to keep up the freshness. The pollen grains were collected from the dehisced anther lobes independently from individual flowers, frozen on the liquid nitrogen and stored them at -195 0 for further studies. For morphological examinations, the individual pollen grains were directly dusted on to the slides and length and breadth of the individual grains were measured using scanning electronmicroscope (TM3000, Hitachi, Japan). The reproductive parts of both male sterile and male fertile flowers and the cross section of the anther lobes were additionally seen under the scanning electronmicroscope (TM3000, Hitachi, Japan),in order to study the morphological difference between the male sterile and male fertile flowers.The stereo microscopy images of the dehisced flowers were additionally examined (ZEISS Stereo zoom microscope Stemi 508 doc, Germany).
5
SEM Analysis of PPE Carriers
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As an internal experimental control, SEM was performed on the PPE carriers TESIMAX® S3 PE-T and TESIMAX® SYKAN 2 before and after treatment to exclude the influence of fabric damage on spore calculation. Small discs (5 mm in diameter) were punched out of the treated and untreated PSA samples using a tissue punch and fixed onto an SEM stub with conductive tape. A thin (5 nm) layer of gold/palladium was generated on the sample surface using a sputter coater (E5100, Polaron). Scanning microscopy was done with a tabletop microscope (TM3000, Hitachi High-Technologies) equipped with a semiconductor backscattered-electron detector at 5 and 15 kV acceleration voltage.
6
Scaffold Macro-Micro Characterization
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To macro-morphologies of the PS and ES scaffolds with the same weight (22 mg) were recorded by a digital camera (D90, Nikon Inc., Melvile, NY, USA). A scanning electron microscope (SEM, TM3000, Hitachi High-Technologies in Europe, Mannheim, Germany) was used to observe the micro-structures of the scaffolds. Samples were first sputter coated with gold and scanned at different magnifications with an acceleration voltage of 25 kV.
7
Scanning Electron Microscopy of Film Cross-Sections
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The film microstructure was observed under a scanning electron microscope, TM3000 tabletop microscope (HITACHI High-Technologies Europe GmbH, Krefeld, Germany). A 5 × 5 mm piece of film was fixed on the support using carbon paste at an angle of 90° to the surface, which allowed observation of the cross-section of the film cut with a scalpel. No particular film preparation was necessary. The films were observed at a magnification of ×1500 (surfaces) and ×1800 (cross-sections).
8
Scanning Electron Microscopy of Ceramic-Resin Failure
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The failure mode of the specimens was analyzed by scanning electron microscopy (SEM) (TM3000, Hitachi High-Technologies Co.; Tokyo, Kantō, Japan) at 200x magnification, at an acceleration voltage of 25 kV. The failure modes were classified as: I- adhesive failure; II- cohesive failure at resin cement; III- cohesive failure at ceramic substrate; IV- mixed failure with predominance of resin cement; V- mixed failure with predominance of ceramic substrate. Adhesive failure was characterized as a fault at the junction between the ceramic and the resin cement; cohesive failure when there was a fracture in the body of the cement or the ceramic; and mixed failure when there was cohesion failure in both materials, simultaneously.
9
Deposition of Gold Nanoparticles on Silicon Nitride Membranes
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Specimens were prepared by dispersing gold colloidal particles with diameters of 50 nm (British Biocell International Solutions, UK) on a silicon nitride membrane with a thickness of 100 nm17 (link). To increase the affinity of the particles to the membrane, the membrane was coated with PLL after the deposition of a thin carbon layer with an approximate thickness of 30 nm. To uniformly disperse gold colloidal particles, we used an electrospray device (PDS-D01, Hamamatsu Nano Technology, Japan). A suspension of gold colloidal particles is applied into a thin capillary tube with an exit nozzle of an inner diameter of 24 μm. The voltage between the electrode inside the capillary and the membrane was adjusted to 5000 V. It took approximately 30 min until the number density reached approximately 50 particles/2 × 2 μm2 on the membrane. After the preparation, the number density was checked by using a scanning electron microscope (SEM) (TM3000, Hitachi High-Technologies, Japan).
10
Evaluating Cell Response to α-TCP Cements
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In order to analyse impact of α-TCP-based cements on cells cultured directly on their surface, cell morphology was analyzed by scanning electron microscopy. Samples of cements were pre-incubated for 1 h in culture medium, then medium was removed and samples were placed into 24-well culture plate. 25 μl of cell suspension with 20 000 MG-63 cells was pipetted onto each sample surface, cells were allowed to adhere for 1 h, then volume of culture medium was filled up to 1 ml and in vitro culture was continued for 48 h. Afterwards samples were washed with PBS (Life Technologies), fixed and dehydrated. Observation was done in TM3000 (Hitachi High-Technologies, Japan) scanning electron microscope.
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