Both thighs as well as the abdomen of the rat were shaved. On both sides, the sciatic nerve was exposed carefully. The vasculature of the lower extremity was preserved by aortic infusion19 . A long longitudinal cut was made medially to expose the vena cava and aorta and these were cleaned from debris. These vessels were ligated as proximal as possible using a 5–0 Vicryl suture (Vicryl Rapide, Ethicon Inc., Sommerville, NJ, USA). Distal to the ligation, a catheter was inserted in the aorta. A yellow Microfil® compound (MV 8ml, diluent 15 ml, and curing agent 1.2 ml, Flow Tech, Inc., Carver, MA, USA) was infused into the aorta. After the contrast agents had cured, bilateral sciatic nerves were harvested. Nerves were temporarily stored in PBS and cleared in graded series of ethyl alcohol (25%, 50%, 75%, 95%, 100%) and placed in methyl salicylate. Clearing the nerve tissue while preserving the injected Microfil® allowed for measurement of the vascularity of the nerve segments.
Microfil compound
Manufactured by Flow Tech
Sourced in United States
Microfil® compound is a laboratory equipment product designed for filtration applications. It is a compound material used in the construction of filtration devices to facilitate the separation of solid particles from liquids or gases.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using microfil compound
1
Microfil Vascular Imaging of Rat Sciatic Nerve
2
Vascular Perfusion Technique for Micro-CT Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
At appropriate time points, animals were euthanized and PBS was perfused through the left ventricle until there was negligible blood detectable in the atrial vent. MICROFIL compound (Flow Tech Inc) was then prepared by adding an appropriate curing agent, after which this compound was infused via the ventricular cannula until the compound was observed to freely flow out of the atrial vent. At this point, clamps were used to close both the ventricular and atrial cannulas, and animals were moved to 4°C overnight. The following day, brains were dissected from the skull, keeping the dura mater intact, and were added to a 4.5% formalin solution for 24 hours prior to micro‐CT examination.
3
Whole-Heart Coronary Vasculature Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
To image the entire coronary vasculature, hearts were washed and retrograde-perfused via the thoracic aorta with Microfil compound (Flow Tech Inc.), previously described but modified.
40 (link)
For optical clearing, hearts were incubated in methyl salicylate (Sigma Aldrich) and afterward imaged under a stereomicroscope (Leica MZ6 with Leica KL 1500 LCD).
+ Expand
To image the entire coronary vasculature, hearts were washed and retrograde-perfused via the thoracic aorta with Microfil compound (Flow Tech Inc.), previously described but modified.
40 (link)
For optical clearing, hearts were incubated in methyl salicylate (Sigma Aldrich) and afterward imaged under a stereomicroscope (Leica MZ6 with Leica KL 1500 LCD).
4
Microfil Perfusion for Angiogenesis Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To investigate new blood vessels formation in defect area, microfil compound (Flowtech, USA) was perfused at 8-week post-operation. Rib cage was opened to expose the heart after animal anesthetized deeply. Heparinized normal saline 20 mL was perfused (100 U/ml, Aladdin, China) firstly, then microfil compound was slowly perfused. Perfused rats were stored at 4 °C overnight, then the calvaria was harvested and fixed in formalin [21 ].
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!