Supernatant was collected from MPC cultures 1 and 8 days after siRNA transfection. BDNF concentration was quantified with the Human/Mouse BDNF DuoSet ELISA kit (R&D Systems), using an Infinite M200 Pro microplate reader (Tecan).
Human mouse bdnf duoset elisa kit
Manufactured by R&D Systems
Sourced in United States
The Human/Mouse BDNF DuoSet ELISA kit is a sandwich enzyme-linked immunosorbent assay (ELISA) designed for the quantitative measurement of brain-derived neurotrophic factor (BDNF) levels in human and mouse biological samples.
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Lab products found in correlation
2 protocols using human mouse bdnf duoset elisa kit
1
BDNF Quantification in MPC Cultures
2
BDNF Quantification in Tissue Samples
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BDNF was measured using the Human/Mouse BDNF DuoSet ELISA Kit (R&D Systems, Minneapolis, MN, US, Cat# DY248) according to manufacturer’s instructions. Tissue samples were diluted 4× for BDNF measurements; results are presented as the mean of two parallels for each sample. The absorbance of BDNF was read at 450 nm. Tissue levels of BDNF were expressed as pg mg−1.
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