Four microorganisms strains were tested: Gram-positive bacteria (Staphylococcus aureus ATCC 6538-P), Gram-negative bacteria (Klebsiella pneumonia ATCC 10031, Escherichia coli ATCC 8739), and Candida albicans ATCC 10231.
Sensitivity studies of microorganisms were performed on standard nutrient media:
To prepare suspensions of microorganisms of the desired concentration, a DEN-1 densitometer was used to measure the optical density (turbidity). Suspensions of microorganisms were prepared on a saline solution of sodium chloride (0.9% NaCl). 5 ml of saline solution was added to the test tube, which was placed in a densitometer, and the optical density was measured. First, a suspension of microorganisms with a concentration of 1.5 × 108 CFU/ml for bacteria was prepared, which corresponds to turbidity of 0.5 units according to McFarland; tenfold dilutions were made from these suspensions, transferring 1.0 ml of the suspension in 9.0 ml of sterile saline solution. Thus, a dilution of 1.5 × 106 CFU/ml for bacteria was obtained. For mushrooms, the suspension was prepared in the same way. The density of the fungi suspension corresponded to 2.0 × 106 CFU/ml.
Sensitivity studies of microorganisms were performed on standard nutrient media:
Mueller Hinton Agar (М173), HiMedia, India
Mueller Hinton Broth (М391), HiMedia, India
Fluid Sabouraud medium (M013), HiMedia, India
To prepare suspensions of microorganisms of the desired concentration, a DEN-1 densitometer was used to measure the optical density (turbidity). Suspensions of microorganisms were prepared on a saline solution of sodium chloride (0.9% NaCl). 5 ml of saline solution was added to the test tube, which was placed in a densitometer, and the optical density was measured. First, a suspension of microorganisms with a concentration of 1.5 × 108 CFU/ml for bacteria was prepared, which corresponds to turbidity of 0.5 units according to McFarland; tenfold dilutions were made from these suspensions, transferring 1.0 ml of the suspension in 9.0 ml of sterile saline solution. Thus, a dilution of 1.5 × 106 CFU/ml for bacteria was obtained. For mushrooms, the suspension was prepared in the same way. The density of the fungi suspension corresponded to 2.0 × 106 CFU/ml.