Apc cy7 conjugated anti mouse cd11b

Manufactured by BD

APC-Cy7 conjugated anti-mouse CD11b is a fluorescently labeled antibody that binds to the CD11b surface antigen expressed on mouse cells. It can be used for the identification and analysis of mouse cells by flow cytometry.

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Lab products found in correlation

Fitc conjugated anti mouse cd3, by Thermo Fisher Scientific (1 mentions) Pe conjugated anti mouse cd19, by BD (1 mentions) Pe conjugated anti mouse ly6g, by BD (1 mentions) Diphtheria toxin, by Merck Group (1 mentions) Percp conjugated anti mouse cd4, by BD (1 mentions) Mouse th1 th2 th17 cytokine kit, by BD (1 mentions) Calcein blue, by Thermo Fisher Scientific (1 mentions) Propidium iodide, by Thermo Fisher Scientific (1 mentions) Apc cy7 conjugated anti mouse ter119, by BioLegend (1 mentions) Biotin rat anti mouse cd184, by BD (1 mentions) Pe cy7 conjugated streptavidin, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

APC-Cy7-conjugated anti-mouse CD11b (clone M1/70, APC-Cy7-conjugated anti-CD11b APC-Cy7-anti-mouse-CD11b APC-conjugated anti-mouse CD11B Anti-CD11b-APC-Cy7 APC-conjugated anti-CD11b CD11b-APC-Cy7 APC anti-mouse CD11b Anti-CD11b-APC Mouse anti-CD11b

2 protocols using apc cy7 conjugated anti mouse cd11b

Multiplex Immunophenotyping of Immune Cells

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The following antibodies were purchased from eBioscience: Pacific Blue conjugated anti-mouse CD4 (clone: RM4-5), FITC conjugated anti-mouse CD45.1 (clone: A20), biotin conjugated anti-mouse CD3 (clone: 17A2), FITC conjugated anti-mouse CD3 (clone: 145-2C11), APC conjugated anti-mouse CD317 (BST2, PDCA1) (clone: eBio927), Pacific Blue conjugated anti-mouse CD11c (clone: N418), FITC conjugated anti-mouse MHCII (I-A) (clone: NIMR-4), biotin conjugated CD317 (BST2, PDCA1) (clone: eBio927), PerCP-Cy5.5 conjugated anti-mouse Ly-6G (Gr-1) (clone: RB6-8C5), streptavidin conjugated PerCP-Cy5.5. The following antibodies were from BD Pharmingen: PE-Cy7 conjugated anti-mouse CD45 (clone: 30-F11), PerCP conjugated anti-mouse CD4 (clone: RM4-5), PE conjugated anti-mouse CD19 (clone: 1D3), APC-Cy7 conjugated anti-mouse CD11b (clone: M1/70), Biotin conjugated anti-mouse Ly-6C (clone: AL-21), PerCP-Cy5.5 conjugated anti-mouse CD45R (B220) (clone: RA3-6B2), PE conjugated anti-mouse Ly-6G (clone: 1A8), PerCP-Cy5.5 conjugated Ly-6C (clone: AL-21), streptavidin conjugated V500. Diphtheria toxin (DT, isolated from Corynebacterium diphtheriae) was purchased from Sigma-Aldrich (St Louis, MO). Mouse Th1/Th2/Th17 Cytokine kit (BD Biosciences, California, USA) was obtained from BD^TM Cytometric Bead Array (CBA).

Rahman T., Brown A.S., Hartland E.L., van Driel I.R, & Fung K.Y. (2019). Plasmacytoid Dendritic Cells Provide Protection Against Bacterial-Induced Colitis. Frontiers in Immunology, 10, 608.

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Isolation of Muscle and Tumor Cell Subsets

Cited 1 time

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Phenotypically distinct muscle and tumor cell subsets were sorted according to protocols that were previously established to isolate functionally discrete subsets of myofiber-associated cells [10 (link), 11 (link), 1 (link)]. In brief, cells were suspended in HBSS supplemented with 2% FBS. Antibody staining was performed for 20 minutes on ice. The following primary and secondary antibodies were used: APC-CY7-conjugated anti-mouse CD11b (1 in 200, BD Pharmingen, 557657), APC-CY7-conjugated anti-mouse CD45 (1 in 200, eBioscience, 557659), APC-CY7-conjugated anti-mouse TER119 (1 in 200, BioLegend, 116223), APC-conjugated anti-mouse Sca1 (1 in 200, eBioscience, 17-5981-82), PE-conjugated anti-mouse/ rat CD29 (1 in 400, BioLegend, 102207), biotin rat anti-mouse CD184 (1 in 100, BD Pharmingen, 551968), PECY7-conjugated Streptavidin (1 in 200, eBioscience, 25-4317-82). Antibody staining was performed for 20 minutes on ice. Prior to FACS sorting, cells were suspended in 1µg/ml propidium iodide and 10µM calcein blue (Invitrogen) to identify viable cells (Pi⁻Ca⁺). Cells were sorted twice to maximize purity.

Hettmer S., Lin M.M., Tchessalova D., Tortorici S.J., Castiglioni A., Desai T., Mao J., McMahon A.P, & Wagers A.J. (2015). Hedgehog-driven myogenic tumors recapitulate skeletal muscle cellular heterogeneity. Experimental cell research, 340(1), 43-52.

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