Anti hsp70 antibody

Male C57BL/6J mice (aged 8 weeks) were obtained from Vital River Laboratory Animal Technology (Beijing, China). All mice were housed in a facility with a 12‐hour/12‐hour light/dark cycle and given free access to water and standard rodent chow. All animal protocols conformed to the Guidelines for the Care and Use of Laboratory Animals, prepared and approved by the Animal Care and Use Committee of the Chinese Academy of Medical Sciences and Peking Union Medical College. HF was induced in 10‐week‐old male C57BL/6J mice by a single IP injection of doxorubicin at a dose of 15 mg/kg. The mice were treated with anti‐HSP70 antibody (Thermo; MA3‐009), isotype‐matched IgG antibodies, or saline on days 10, 14, 18, 25, and 32 after doxorubicin injection (n=60 for saline or doxorubicin group; n=40 for HSP70 antibody or IgG antibody group). The dosing and timing of antibodies were determined on the basis of our previous studies and pilot experiment.15 The antibodies were dissolved in sterile saline and administered by tail vein injection. The first dose was 200 mg/kg, and the remaining doses were 100 mg/kg. Six mice per group were euthanized by an overdose of sodium pentobarbital injection (IP, 100 mg/kg), and the hearts were harvested and sliced into several sections on day 1, 3, 7, 11, 13, 17, 24, and 36 for the indicated analyses, as described below.