Visualization through positive-negative staining was performed as described in Théry et al. 200622 on a Hitachi 7700 transmission electron microscope (Tokyo, Japan). Methods for immunogold labeling are included in supplemental materials .
7700 transmission electron microscope
Manufactured by Hitachi
Sourced in Japan
The Hitachi 7700 Transmission Electron Microscope (TEM) is a high-performance instrument designed for advanced materials analysis. It features a high-resolution electron optical system and provides clear, detailed images of samples at the nanoscale level. The 7700 TEM is capable of performing a wide range of analytical techniques, including imaging, diffraction, and spectroscopy, to support comprehensive materials characterization.
Automatically generated - may contain errors
Lab products found in correlation
Poly bed 812 resin, by Polysciences (2 mentions)
Uranyless, by Electron Microscopy Sciences (2 mentions)
Uc7 ultramicrotome, by Leica (2 mentions)
Copper grid, by Merck Group (2 mentions)
Ix71 microscope, by Olympus (1 mentions)
Ti microscope, by Nikon (1 mentions)
Fluorolog 3 spectrofluorometer, by Horiba (1 mentions)
D max 2550 x ray diffractometer, by Rigaku (1 mentions)
Zen 3600 zetasizer, by Malvern Panalytical (1 mentions)
Fois 1 integrating sphere, by OceanOptics (1 mentions)
Fs5 spectrometer, by Edinburgh Instruments (1 mentions)
Fv1200, by Olympus (1 mentions)
Nicolet is5 ftir spectrometer, by Thermo Fisher Scientific (1 mentions)
Microtome, by Leica (1 mentions)
Formvar carbon coated copper grid, by Electron Microscopy Sciences (1 mentions)
Fluoview fv1000 laser confocal microscopy system, by Olympus (1 mentions)
Digital micrograph software, by Ametek (1 mentions)
Fluoview fv1000, by Olympus (1 mentions)
P6148, by Merck Group (1 mentions)
Sc 7637, by Santa Cruz Biotechnology (1 mentions)
31 protocols using 7700 transmission electron microscope
1
Visualization of Extracellular Vesicles
2
Characterization of Ag-PMMA Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ultraviolet–Visible spectroscopy. A UV/Vis spectrophotometer model T80 (Pg Instruments Ltd) was employed to study: a) the optical characteristics of the AgNP-PMMA colloidal solution by recording its absorbance in the range between 300 and 800 nm; b) the sensor response to different concentrations of various analytes. These measurements were carried out for each analyte by monitoring the absorbance variation with time (30 min) at 416 nm; c) the release of MMA monomers into the water solution used for the heat treatment of PMMA by measuring its absorption spectra in a range between 190 and 350 nm; d) the change in the optical characteristics of a PMMA sheet by recording its UV-Vis spectra before and after the thermal treatment.
Transmission Electron microscopy. TEM images and electron diffraction patterns were taken using an Hitachi 7700 transmission electron microscope operated at 100 kV. This acceleration voltage was settled to obtain a sufficient resolution and minimal radiation damage of the material. Specimens for TEM observations was prepared by drop-casting of freshly solutions containing Ag-PMMA nanoparticles onto standard carbon supported 600-mesh copper grid and drying slowly in air naturally.
Transmission Electron microscopy. TEM images and electron diffraction patterns were taken using an Hitachi 7700 transmission electron microscope operated at 100 kV. This acceleration voltage was settled to obtain a sufficient resolution and minimal radiation damage of the material. Specimens for TEM observations was prepared by drop-casting of freshly solutions containing Ag-PMMA nanoparticles onto standard carbon supported 600-mesh copper grid and drying slowly in air naturally.
3
Renal Cortex Ultrastructural Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Renal cortex tissues were fixed with 2.5% glutaraldehyde for 2 h at room temperature and transferred to 4°C for storage. After dehydration in ethanol, the specimens were embedded in EPON. Ultrathin sections were photographed under a Hitachi 7700 transmission electron microscope (Tokyo, Japan).
4
Kernel Development Analysis Workflow
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Wild-type and zmnmat1 mutant kernels at 9 DAP and 15 DAP were collected from the same heterozygous ears, cut along the longitudinal axis, and then fixed in FAA solution (5 mL 37% formaldehyde, 90 mL 70% ethanol, and 5mL glacial acetic acid) for paraffin section preparation. The fixed samples were firstly embedded in paraffin, then cut into 8 μm sections, stained with toluidine blue, and observed under a Nikon Ti Microscope (Nikon, Tokyo, Japan) as described previously (Ren et al., 2019 (link)). For resin sections, samples at 15 DAP were fixed in 2.5% glutaraldehyde, immersed in resin, then cut into 1.4 μm sections, and observed under an Olympus IX71 Microscope. Ultrathin sections of TEM were observed under a Hitachi 7700 Transmission Electron Microscope.
5
Structural and Optical Characterization of UC Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
XRD patterns were obtained by a Rigaku D/max 2550 X-ray diffractometer using CuKa radiation (λ = 0.154 nm). TEM images were performed on a Hitachi 7700 transmission electron microscope operating at 200 kV. UC luminescence spectra and decay curves were recorded on a Fluorolog®-3 Spectrofluorometer by Horiba using an external (continuous or pulsed) 980 nm diode laser as the excitation source. All measurements were taken under room temperature.
6
Negative-Positive EV Staining and Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Negative-positive EV staining was performed as described in (30 (link)). Briefly, EVs were thawed and fixed with glutaraldehyde, before adding the EVs to formvar-coated copper grids by inversion of grids on EV droplets for 20 minutes. Following a series of washes, EVs were negative stained with methylcellulose-uranyl acetate, followed by positive staining with uranyl oxalate. Grids were left to dry for at least two hours before imaging using a Hitachi 7700 transmission electron microscope (Tokyo, Japan).
7
Ultrastructural Analysis of Mouse Spinal Cord
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After anesthetization, mice were perfused with normal saline. The lumbar enlargements were harvested and incubated with 2.5% glutaraldehyde. Samples were post-fixed with 1% osmium tetroxide and dehydrated in a graded series of diluted acetone solutions. After embedding in epoxy resin, samples were cut into ultrathin sections and stained with uranyl acetate and citric acid lead. Images were captured using a Hitachi 7700 transmission electron microscope.
8
Ultrastructural Analysis of Rat Podocytes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Several 1-mm cubes from cortex of left kidneys were cut, placed in 2.5% glutaraldehyde for at least 4 h washed with cacodylate buffer, postfixed in 1% osmium tetroxide and block-stained in uranyl acetate before embedding in Poly/Bed812 resin (Polysciences, Inc., Warrington, PA, USA). Ultrathin sections were obtained from at least three randomly selected glomeruli from each animal, stained with uranyl acetate and lead citrate and examined using a Hitachi 7,700 transmission electron microscope (Tokyo, Japan). Representative micrographs of the podocytes from each rat were examined and imaged at a magnification of × 4000.
9
Visualization of α-Synuclein by TEM
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Samples (30 μl) of a 14 μM α-synuclein solution were adsorbed onto glow-discharged 200 mesh formvar carbon coated copper grids (Electron Microscopy Sciences) and stained with aqueous uranyLess (Electron Microscopy Sciences). Excess liquid was removed and grids were allowed to air dry. Samples were viewed using a Hitachi 7700 transmission electron microscope.
10
Optical Characterization of Quantum Dots
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Transmission electron microscopic (TEM) images were taken using a Hitachi 7700 transmission electron microscope (Tokyo, Japan) with an accelerating voltage of 120 kV. The steady-state and transient photoluminescence spectra were measured using an Edinburgh Instruments FS5 spectrometer (Livingston, UK) with the excitation wavelengths at 405 nm and 450 nm, respectively. The absolute photoluminescence quantum yield of the QDs was measured using a QE65000 spectrometer equipped with an Ocean Optics FOIS-1 integrating sphere. The QD sample was gradiently diluted with an optical density below 0.1. Each diluted sample was measured at room temperature. The accuracy and stability of the measurement system were verified using organic dyes with known photoluminescence quantum yields. The ultraviolet-visible (UV-vis) spectra were monitored using an AgilentCary 60 spectrophotometer (Palo Alto, CA, USA). The zeta potential and hydrated particle size were measured at a neutral pH with a Malvern Zen 3600 Zetasizer (Malvern, UK). All the optical measurements were performed at room temperature.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!