To check the purity of isolated T cells using magnetic beads, isolated T cells were stained with APC-conjugated anti‐human CD3 (Clone: UCHT1, BioLegend). Mesothelin expression was detected using PE-conjugated anti-human mesothelin (Clone: #420411, R&D Systems). All samples were acquired with a BD FACSCalibur (BD Biosciences) and analyzed using FlowJo software (v10.6). All assays were performed in duplicate and repeated two to three times.
Pe conjugated anti human mesothelin
Manufactured by R&D Systems
PE-conjugated anti-human mesothelin is a laboratory reagent used to detect and quantify the expression of mesothelin, a cell surface glycoprotein, in human samples. It consists of a phycoerythrin (PE) fluorescent label conjugated to an antibody specific for the human mesothelin protein.
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Lab products found in correlation
Apc conjugated anti human cd3, by BioLegend (2 mentions)
Facscalibur, by BD (2 mentions)
Pe conjugated anti human cd279 pd 1, by BioLegend (1 mentions)
Percp conjugated anti human cd3 antibody, by BioLegend (1 mentions)
Fitc conjugated anti human cd3, by BioLegend (1 mentions)
Celltrace cfse, by Thermo Fisher Scientific (1 mentions)
2 protocols using pe conjugated anti human mesothelin
1
T Cell Purity Validation Using Flow Cytometry
2
Characterization of Activated T Cell Phenotype
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The purity of isolated T cells was confirmed using APC-conjugated anti-human CD3 (Clone: UCHT1, BioLegend). PE-conjugated anti-human mesothelin (Clone: #420411, R&D Systems) was used to detect mesothelin expression. FITC-conjugated anti-human CD3 (Clone: HIT3a, BioLegend), PE-conjugated anti-human CD279 (PD-1) (Clone: EH12.2H7, BioLegend), and APC-conjugated anti-human CD366 (Tim-3) (Clone: F38-2E2, BioLegend) antibodies were used to measure the expression of exhaustion markers. For proliferation assays, cells were loaded with CellTrace™ CFSE (Life Technologies, #C34554) according to manufacturer’s instructions, and T cells were detected using PerCP-conjugated anti-human CD3 antibody (Clone: HIT3a, BioLegend). Data were collected using a BD FACSCalibur (BD Biosciences) and analyzed with FlowJo software (v10.6). All assays were performed in duplicate and repeated two to three times.
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