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Cpg a

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CpG-A is a synthetic oligodeoxynucleotide that contains unmethylated CpG motifs. It is designed to stimulate the innate immune response by activating Toll-like receptor 9 (TLR9).

Automatically generated - may contain errors

Lab products found in correlation

Cpg b, by InvivoGen (9 mentions) Poly 1 c, by InvivoGen (5 mentions) Lipopolysaccharide lps, by InvivoGen (5 mentions) Imiquimod, by InvivoGen (4 mentions) L glutamine, by Corning (4 mentions) Sodium pyruvate, by Corning (4 mentions) Roswell park memorial institute (rpmi), by Corning (4 mentions) Hepes, by Corning (4 mentions) Mem non essential amino acid, by Corning (4 mentions) 2 mercaptoethanol, by Thermo Fisher Scientific (4 mentions) Streptomycin, by Corning (4 mentions) Penicillin, by Corning (4 mentions) Lipopolysaccharides (lps), by InvivoGen (3 mentions) Pam3csk4, by InvivoGen (3 mentions) Polyinosinic polycytidylic acid, by InvivoGen (3 mentions) Rpmi 1640, by Thermo Fisher Scientific (3 mentions) Resiquimod r848, by InvivoGen (3 mentions) Cgamp, by InvivoGen (3 mentions) Cl097, by InvivoGen (2 mentions) Dotap, by Santa Cruz Biotechnology (2 mentions)

Spelling variants of this product

CpG-A ODN2216 (7 citations) CpG-A (ODN1585) (4 citations) CpG A 1585 (3 citations) CpG-A (2216; (3 citations) Class‐A CpG oligonucleotide ODN‐2336 (CpGA, (3 citations) Murine CpG oligodeoxynucleotide (CpG) TLR9 ligand (ODN 1585; Class A) (3 citations) CpG-A oligodeoxynucleotide 1585 (2 citations) Class‐A CpG oligonucleotide ODN‐2336 (2 citations)

37 protocols using cpg a

1

Evaluating pDC Responses to TLR Stimuli

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When assessing the impact of γδ T cells on pDCs, the ability of pDCs to respond to a subsequent TLRL stimulation was assessed following the first coculture. In this case, the γδ T cells-pDCs cocultures were harvested after 20 h, washed, counted and pDCs were resuspended at 1 × 106/ml and further cultured 24 h in absence or presence of TLR7L (CL097, 1 μg/mL) or TLR9L (CpGA, 1.5 μM) (Invivogen).
Girard P., Ponsard B., Charles J., Chaperot L, & Aspord C. (2020). Potent Bidirectional Cross-Talk Between Plasmacytoid Dendritic Cells and γδT Cells Through BTN3A, Type I/II IFNs and Immune Checkpoints. Frontiers in Immunology, 11, 861.
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2

CpG-A Immune Stimulation Assay

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Two μg of CpG-A (ODN2216; Invivogen) and 12 μL DOTAP (Santa Cruz Biotechnology) were mixed with 100 μL of PBS and administered intravenously (i.v.) by tail vein injection. Control mice received 12 μL DOTAP with PBS. Spleens were harvested and analyzed 6 hours after inoculation.
Leylek R., Alcántara-Hernández M., Lanzar Z., Lüdtke A., Perez O.A., Reizis B, & Idoyaga J. (2019). Integrated Cross-Species Analysis Identifies a Conserved Transitional Dendritic Cell Population. Cell reports, 29(11), 3736-3750.e8.
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3

Neutrophil-Derived Mitochondrial DNA Activation of Plasmacytoid Dendritic Cells

Cited 1 time
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Ox mtDNA was generated as previously described3 (link). Briefly, healthy neutrophils were pre-incubated with IFNα2β (2000 U/ml; Schering Corp.) for 90 min at 37 C and then extensively washed before incubation with anti-RNP IgG (50 μg/ml) purified from SLE patient sera. Neutrophil supernatants were then collected, centrifuged for 10 min at 1400g and stored at −80 C. PDCs (5×105 cells/well – 96 U bottom plate) were cultured with 40% v/v Ox mtDNA-containing neutrophil supernatants (referred in the text as “Ox mtDNA”) or with 5 μg/ml of either CpGA (ODN-2216; Invivogen) or CpGB (ODN-2006; Invivogen) for 24 h. The volume of Ox mtDNA-containing neutrophil supernatants and the concentration of CpGA were selected based on their capacity to trigger similar levels of IFNα production by pDCs.
Caielli S., Troggian Veiga D., Balasubramanian P., Athale S., Domic B., Murat E., Banchereau R., Xu Z., Chandra M., Chung C.H., Walters L., Baisch J., Wright T., Punaro M., Nassi L., Stewart K., Fuller J., Ucar D., Ueno H., Zhou J., Banchereau J, & Pascual V. (2018). A CD4+ T cell population expanded in Lupus blood provides B cell help through IL10 and succinate. Nature medicine, 25(1), 75-81.
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4

Immune Stimulant Compounds Protocol

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LTA-SA, FSL-1, FLA-ST, poly(I:C), LPS, R837, R848, CpG-A (ODN 2216) and CpG-B (ODN 2006) were purchased from Invivogen.
Chow K.T., Wilkins C., Narita M., Green R., Knoll M., Loo Y.M, & Gale M J.r. (2018). Differential and overlapping immune programs regulated by IRF3 and IRF5 in plasmacytoid dendritic cells. Journal of immunology (Baltimore, Md. : 1950), 201(10), 3036-3050.
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5

Assessing pDC Activation after γδ T Cell Coculture

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After coculture with γδ T cells, the ability of pDCs to respond to a subsequent TLRL stimulation was assessed following the first coculture. In this case, the γδ T cells–pDCs cocultures were harvested upon 20 h, washed and counted, and pDCs were resuspended at 1 × 106 mL−1 and further cultured 24 h in the absence or presence of TLR7‐L (CL097, 1 μg mL−1) or TLR9L (CpGA, 1.5 μm) (Invivogen).
Girard P., Sosa Cuevas E., Ponsard B., Mouret S., Gil H., Col E., De Fraipont F., Sturm N., Charles J., Manches O., Chaperot L, & Aspord C. (2021). Dysfunctional BTN3A together with deregulated immune checkpoints and type I/II IFN dictate defective interplay between pDCs and γδ T cells in melanoma patients, which impacts clinical outcomes. Clinical & Translational Immunology, 10(11), e1329.
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6

CpG-A Immune Stimulation Assay

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Two μg of CpG-A (ODN2216; Invivogen) and 12 μL DOTAP (Santa Cruz Biotechnology) were mixed with 100 μL of PBS and administered intravenously (i.v.) by tail vein injection. Control mice received 12 μL DOTAP with PBS. Spleens were harvested and analyzed 6 hours after inoculation.
Leylek R., Alcántara-Hernández M., Lanzar Z., Lüdtke A., Perez O.A., Reizis B, & Idoyaga J. (2019). Integrated Cross-Species Analysis Identifies a Conserved Transitional Dendritic Cell Population. Cell reports, 29(11), 3736-3750.e8.
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7

Dendritic Cell Vaccination with TLR Agonists

Cited 1 time
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Bone marrow-derived CD11c+ DCs were generated after 5 days of culture with GM-CSF and matured with LPS (50ng/ml) overnight as described previously (11 (link), 30 (link)). Matured DCs were pulsed with GP33-41 peptide (200ng/ml) for 2h, washed with PBS and 1×106 DC-33 cells were injected intravenously (i.v.). Simultaneously, DC immunized mice were injected intraperitoneally (i.p.) with either PBS or one of the following TLR ligands: PAM3CSK4 (50μg), poly I:C (50μg), LPS (20μg), MPLA (30μg), Flagellin(20μg), LTA(20μg), R848 (20μg), CpG-A (50μg) or CpG-B (50μg). The dose for each adjuvant was primarily determined by either previous publications (14 (link), 15 , 31 (link)-34 (link)) and/or manufacture recommendations. Additional titrations were done in some cases to ensure comparable CD8 T cell responses at the effector phase as shown in Supplementary Figure 1 and no perceivable side effects. LPS was purchased from Sigma-Aldrich, St. Louis, MO; CpG-A and CpG-B were synthesized at Yale University Keck Facility; and the rest were all purchased from InvivoGen, San Diego, CA.
Cui W., Joshi N.S., Liu Y., Meng H., Kleinstein S.H, & Kaech S.M. (2014). TLR4 ligands LPS and MPLA differentially regulate effector and memory CD8+ T cell differentiation. Journal of immunology (Baltimore, Md. : 1950), 192(9), 4221-4232.
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8

PBMC Stimulation and IFN-α Quantification

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1 × 106 thawed PBMCs were incubated at 37 °C and 5% CO2 during 18 h in RPMI with 10% FBS without any stimuli or with 1 µM CpG-A (ODN 2216; InvivoGen). After incubation, cells were pelleted and the supernatants conserved for the subsequent quantification of IFN-α production at −80 °C. The amount of IFN-α in cell culture supernatants was assessed by an IFN-α multisubtype enzyme-linked immunosorbent assay kit (PBL Interferon Source Cat# 41105) according to the manufacturer’s instructions.
Pérez-Gómez A., Vitallé J., Gasca-Capote C., Gutierrez-Valencia A., Trujillo-Rodriguez M., Serna-Gallego A., Muñoz-Muela E., Jiménez-Leon M.D., Rafii-El-Idrissi Benhnia M., Rivas-Jeremias I., Sotomayor C., Roca-Oporto C., Espinosa N., Infante-Domínguez C., Crespo-Rivas J.C., Fernández-Villar A., Pérez-González A., López-Cortés L.F., Poveda E, & Ruiz-Mateos E. (2021). Dendritic cell deficiencies persist seven months after SARS-CoV-2 infection. Cellular and Molecular Immunology, 18(9), 2128-2139.
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9

Measuring IL-6 Responses to TLR Agonists

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DC and myoblasts were harvested and transferred (respectively 50x103 and 10x103 cells/well) to 96-well plates and incubated with LipoPolySaccharide LPS (1 μg/mL), flagellin (10 ng/mL), zymosan (1 μg/mL), CpG-A (10 μg/mL), imiquimod (0.5 μg/mL), poly (I:C) (1 μg/mL), or Pam3CSK4 (100 ng/mL), all from Invivogen, for 16 hr. IL-6 concentration was measured in culture supernatants using a Human IL-6 ELISA MAX Deluxe, according to the manufacturer’s instructions and performing 3 to 4 technical replicates per condition. The results are expressed in picograms per milliliter (pg/mL). Unless otherwise specified in figures or legends, cytokine concentrations in vitro are presented after background subtraction, obtained with cells incubated with vehicle (DMSO or water). In some experiments the TLR9 antagonist ODN TTAGGG (A151) was added 1 hr before addition of stimuli at the concentration of 1 μmol/L.
Hamel Y., Mauvais F.X., Madrange M., Renard P., Lebreton C., Nemazanyy I., Pellé O., Goudin N., Tang X., Rodero M.P., Tuchmann-Durand C., Nusbaum P., Brindley D.N., van Endert P, & de Lonlay P. (2021). Compromised mitochondrial quality control triggers lipin1-related rhabdomyolysis. Cell Reports Medicine, 2(8), 100370.
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10

PBMC Stimulation and Cytokine Assay

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PBMC (0.5–2 × 106 cells) were treated with 24F4A, isotype control mAb, or culture media for 30 min before treatment with TLR agonists CpG-A 10 μM, R848 1 μM (Invivogen San Diego, CA, USA), or 4 μg/ml of ssRNA complexed with pL-Arginine at 37C and 5% C02. After 2 h, cells were treated with 5 μg/ml Brefeldin A for 4 h before staining.
Gardet A., Pellerin A., McCarl C.A., Diwanji R., Wang W., Donaldson D., Franchimont N., Werth V.P, & Rabah D. (2019). Effect of in vivo Hydroxychloroquine and ex vivo Anti-BDCA2 mAb Treatment on pDC IFNα Production From Patients Affected With Cutaneous Lupus Erythematosus. Frontiers in Immunology, 10, 275.
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