Eclipse a1 microscope

All statistical analyses were performed using GraphPad Prism software 8.0 (RRID: SCR_002798) and SPSS 18.0 software (RRID: SCR_002865). Most of the data were analyzed by a two-tailed Student’s t test or one-way ANOVA with Dunnett’s test for pairwise comparisons. Tumor volumes were analyzed by repeated measures of ANOVA. Phase contrast and fluorescence pictures were taken with a Nikon Eclipse A1 microscope. The IHC staining intensity was analyzed by ImageScope software (ImageScope, RRID: SCR_014311). The Wilcoxon signed-rank test was used to compare non-normally distributed data. Bars show the mean ± SD of three independent repeated experiments. Significant differences were accepted if the p-value was < 0.05.

All statistical analyses were performed using graphpad prism software 8.0 (RRID: SCR_002798) (GraphPad Software Inc., San Diego, California, USA) and spss 18.0 software (RRID: SCR_002865) (IBM SPSS Statistics Inc., Chicago, Illinois, China). Most of the data were analyzed by a two‐tailed Student's t‐test or one‐way ANOVA with Dunnett's test for pairwise comparisons. Tumor volumes were analyzed by a two‐tailed paired Student's t‐test. Correlations were analyzed by the Pearson's test. The expression of CDKN1A in paired cancer and adjacent non‐neoplastic tissue was analyzed by a two‐tailed paired Student's t‐test. Phase‐contrast and fluorescence pictures were taken with a Nikon Eclipse A1 microscope (Tokyo, Japan). The IHC staining intensity was analyzed by imagescope software (ImageScope, RRID: SCR_014311). The Wilcoxon signed‐rank test was used to compare paired non‐normally distributed data. Bars show the mean ± SD or SEM of three independent repeated experiments. Significant differences were accepted if the P‐value was < 0.05.