Axiovert observer z1 inverted

Samples were imaged with the Zeiss LSM 510 Meta system combined with the Zeiss Axiovert Observer Z1 inverted microscope and ZEN 2009 imaging software (Carl Zeiss, Inc., Thornwood, NY). Confocal Z-stack and single plane images were acquired utilizing the Plan-Apochromat 20x/NA 0.8 and Fluar 40x/NA1.30 oil objectives; with a diode (405 nm) and an Argon (488 nm) laser sources. Transmitted light was also collected on a separate channel during the image acquisition to provide contrast to the GF structure. Image processing was performed with ZEN 2009 imaging software (Carl Zeiss, Inc., Thornwood,NY).

For confocal immunofluorescence imaging, the primary antibodies were visualized with secondary antibodies tagged with either Alexa Fluor 488 or Alexa Fluor 555 (Invitrogen, Carlsbad, CA.) Images were taken with the Zeiss LSM 510 Metasystem combined with the Zeiss Axiovert Observer Z1 inverted microscope and ZEN 2009 imaging software (Carl Zeiss, Inc., Thornwood, NY). Confocal Z-stack and single plane images were acquired with an Argon (488 nm) and a HeNe (543 nm) laser source. Z-stacks images were acquired using a 20× Plan-Apochromat (NA 0.8) objective, emission band passes of 505–550 nm for the detection of the nApoECFp17 antibody (green channel, Alexa Fluor 488) and 550–600 nm for both the detection of Olig-1 (red channel, Alexa Fluor 555) and α-Syn (red, Alexa Fluor 555). All images displayed are 2-D maximal intensity projections generated acquired Z-stacks. Single plane images were acquired with a 63× Plan-Apochromat oil-immersion objective (NA 1.4) with emission long pass of 505 nm for the detection of the nApoECFp17 antibody (green channel, Alexa Fluor 488) and 550–600 nm for the detection of either Olig-1 or α-Syn (red channel, Alexa Fluor 555).