Immunohistochemical labeling of embryonic brain sections or dissociated neural cells was performed as described previously (Kim et al., 2009 ). The following primary antibodies were used: rabbit anti-MACF1 (Wu et al., 2011 (link)), rabbit anti-MACF1 (Santa Cruz), rabbit anti-phospho-MACF1 (Wu et al., 2011 (link)), chicken anti-nestin (Neuromics), mouse anti-MAP2 (Covance), rabbit anti-Tbr1 (Chemicon), rabbit anti-Cux1 (Santa Cruz), goat anti-Brn1 (Novus Biologicals), rabbit anti-acetyl-α-tubulin (Cell Signaling), mouse anti-α-tubulin (Sigma), and chicken anti-actin (Millipore). Appropriate secondary antibodies conjugated with Alexa Fluor dyes (Invitrogen) were used to detect primary antibodies.
Chicken anti nestin
Manufactured by Neuromics
Sourced in Denmark
Chicken anti-nestin is a primary antibody used for the detection and localization of nestin, an intermediate filament protein expressed in neural stem and progenitor cells. It can be used in various immunodetection techniques, such as immunohistochemistry and Western blotting, to study the distribution and expression of nestin in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti macf1, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti tbr1, by Merck Group (1 mentions)
Goat anti brn1, by Novus Biologicals (1 mentions)
Alexa fluor dye, by Thermo Fisher Scientific (1 mentions)
Mouse anti α tubulin, by Merck Group (1 mentions)
Rabbit anti acetyl α tubulin, by Cell Signaling Technology (1 mentions)
Rabbit anti cux1, by Santa Cruz Biotechnology (1 mentions)
Mouse anti map2, by Fortrea (1 mentions)
Rat anti cd31, by BD (1 mentions)
Anti mouse igg dylight 405, by Jackson ImmunoResearch (1 mentions)
Mouse anti erk1 2, by Cell Signaling Technology (1 mentions)
Anti rabbit igg dylight 405, by Jackson ImmunoResearch (1 mentions)
Anti mouse irdye 680rd, by LI COR (1 mentions)
Rabbit anti ng2, by Merck Group (1 mentions)
Rabbit anti p erk1 2, by Cell Signaling Technology (1 mentions)
Anti rabbit irdye 800cw, by LI COR (1 mentions)
Rabbit anti gfap, by Agilent Technologies (1 mentions)
Mouse anti e cadherin, by BD (1 mentions)
2 protocols using chicken anti nestin
1
Immunolabeling of Embryonic Neural Cells
2
Visualizing Cell-Cell Interactions with Confocal Microscopy
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Images were acquired using an Olympus FLUOVIEW FV1000 confocal microscope. Fluorescence intensity was measured using ImageJ or Olympus FLUOVIEW software. The Pearson’s coefficient for co-localization was measured using Olympus FLUOVIEW software. Commercially available antibodies used for immunoblotting and immunostaining of tissue sections include rabbit anti-MFSD2a (Cell Signaling Technology, Beverly, MA), mouse anti-E-cadherin (cat#610181, BD, Biosciences, San Diego, CA, USA), rabbit anti-NG2 (Millipore), rabbit anti-GFAP (cat#Z0334, DAKO, Glostrup, Denmark), chicken anti-Nestin (cat#CH23001, Neuromics, Minneapolis, MN, USA), rat anti-CD31 (cat#553370, BD Biosciences, San Diego, CA, USA), rat anti-CD34 (GTX28158, GeneTex, Irvine, CA, USA), mouse-anti-Erk1/2 (cat#9107 S, Cell Signaling Technology), and rabbit-anti-pErk1/2 (cat#4370 s, Cell Signaling Technology). Secondary antibodies include anti-rabbit IgG Alexa Fluor-594/488, 1:500 (cat#711-585-152/cat#711-545-152, Jackson labs), anti-rat IgG Alexa Fluor-594, 1:500 (cat#112-585-167, Jackson labs), anti-mouse IgG Dylight-405, 1:500 (Jackson labs), and anti-rabbit IgG DyLight-405,1:500 (cat#711-475-152, Jackson labs). Secondary antibodies used for immunoblotting were IRDye 800CW anti-rabbit, 1:10,000 (LICOR) and IRDye 680RD anti-mouse, 1:10,000 (LICOR).
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